Mempro™ Cell-Free Glycolipid Transfer Protein Production

Creative Biostructure offersadvanced custom Mempro™ protein production services for glycolipid transfer protein (GLTP) using cell-free expression system. Cell-free protein expression system holds many advantages over the protein expression in cell-based expression system. For example, it’s able to express toxic protein as well as the protein with post-translational modifications. Our patent cell-free expression system ensures the high-yield, high-quality glycolipid transfer protein with its nature conformation.

GLTP is a 24 kDa protein presents in many species that catalyzes the transfer of glycolipids through the intracellular membranes. Both sphingoid- and glycerol-based glycolipids can be transferred by GLTP as long as the hydrophobic lipid backbone has beta-linked initial sugar. The activity of GLPT depends on the composition and the properties of the membranes the glycolipid exists, such as the packing of the membrane and whether the membrane consists of sphingomyelin or phosphatidylcholine. All the known GLPTs from different organisms showed a 3-D structure of a double-layered all-α-helical conformation with a ligand-binding site. The binding site consists of a sugar recognition site and a channel for hydrocarbon chain binding. The interaction between glycolipid and GLTP arises from the hydrogen binding and the hydrophobic effect at the binding site. This unique structure differentiates GLTP from other known lipid transfer proteins.

Figure 1. Crystal structure of a bovine GLTP.

Creative Biostructure is highly experienced in high-yield GLTP production using cell-free protein expression systems, we can provide various strategies for Mempro™ cell-free protein production, including:

• Mempro™ Cell-Free Protein Production in E. coli;
• Mempro™ Cell-Free Protein Production in Wheat Germ;
• Mempro™ Cell-Free Protein Production in Rabbit Reticulocyte;
• Mempro™ Cell-Free Protein Production in Baculovirus.

E. coli S30 extract system is a powerful tool for GLTP production. It simplifies the process of transcription and translation from the DNA to protein. Mempro™ Cell-Free system developed by Creative Biostructure is deficient of protease which ensure the more stable protein production. GLTP has been expressed in E. coli as a GST-fusion protein successfully. E. coli S30 extract system will definitely guarantee the GLTP production with high purity and activity.

With the Mempro™ cell-free protein production platform, Creative Biostructure is capable of expressing, purifying and crystallizing GLTP to facilitate the study of their biological functions.

We provide other various Mempro™ membrane protein production services. Please feel free to contact us for a detailed quote.

References:
F. Bernhard, et al. (2013). Cell-free expression - making a mark. Cur. Opin. Struct. Biol., 23: 374-380.
Lin X, Mattjus P, Pike H M, et al. Cloning and expression of glycolipid transfer protein from bovine and porcine brain[J]. Journal of Biological Chemistry, 2000, 275(7): 5104-5110.
Mattjus P. Glycolipid transfer proteins and membrane interaction[J]. Biochimica et Biophysica Acta (BBA)-Biomembranes, 2009, 1788(1): 267-272.