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Creative
BioStructure
is specialized in providing contract Structural Biology services to both academia and biotechnology/pharmaceutical industry. Creative BioStructure offers the most cost-effective
X-ray Crystallography
and
NMR services
for determining three-dimensional structures of proteins and other macromolecules. Our unsurpassed capacity in the field allows a full range of services including gene expression, protein purification, macromolecular crystallization, X-ray characterization of crystals, processing and quality analysis of data, structure determination, molecular modeling, molecular docking and structure visualization and analysis. Stage-wise services such as crystallization screening, in-house X-ray diffraction data collection and structure determination are also available upon request. In particular, we have extensive experience in co-crystallization of a large number of protein kinases with small molecule compounds. Our expertise permits us to offer first class services at very competitive price.
Creative
BioStructure
is equipped with state-of-the-art fermentation systems for production of recombinant protein in E. coli, yeast, baculovirus-insect cells, and mammalian cells. We will work with our customers to determine the best protocol for a target protein. Our laboratory is equipped with both traditional chromatography systems and HPLC for large-scale protein purification. We test all purified proteins for purity, aggregation state and activity using enzyme assays, dynamic light scattering, UV spectrometry, mass spectrometry and amino acid sequencing.
We can test the crystallization conditions for freshly purified target proteins and compounds provided by clients. We search for both conditions that generate co-crystals (protein plus ligand) and those produce apo-crystals for soaking with ligand. If crystals are obtained, we will fine-tune the conditions to generate diffraction-grade crystals. We collect X-ray diffraction data using our powerful in-house X-ray generator and send the data collected to our customers.
We have extensive experience in structure determination and can provide model building and refinement services based on the X-ray diffraction data collected. If the target protein has homologues with known 3-D structure in the RCSB Protein Data Bank (http://www.rcsb.org/pdb/home/home.do), our crystallographers can resolve the structure by one good set of diffraction data with Molecular Replacement (MR) method. In contrast, for de novo structures, phase problem can be determined by either Multiple Isomorphous Replacement (MIR) using heavy atoms or Multiple Wavelength Anomalous Dispersion (MAD) using selenomethionine derivatives. Depending on the phasing methods, more than one data set is collected. These two methods for novel structures are more costly. Importantly we also have access to facilities that collect data using synchrotron sources. As evidenced by quite a few publications by our staff crystallographers in top journals like Nature and Science, we are professional in resolving the structure of membrane proteins and other difficult proteins as well.
Creative
BioStructure
has also established itself as the leading service provider for structure-based drug discovery and drug development. We also have one division focusing on computer aided drug design. We have developed more than 10 software programs based on proprietary algorithms. We have also constructed a virtual compound database with more than 3.2 million unique compounds, which enables us to pursue in silico high throughput drug screening. Our Structure Biology platform is well backed by the unparalleled expertise available at our sister companies in the field of protein expression [Creative Biolabs], Fab based conformational stabilization in crystallization using phage display technology [Creative Biolabs], Medicinal Chemistry [BOC Sciences] and preclinical research [Creative Animodel]; as a result, we are able to offer a wide range of capabilities required in the entire structure based drug development process.
We have set up our headquarters in Long Island, New York, where we have immediate access to research facilities of Stony Brook University. Besides, we also benefits from the synchrotron resource at Brookhaven National Laboratories (BNL) and Molecular Biology facility at Cold Spring Harbor Laboratories (CSHL). |
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Knowledge-based design of protein expression constructs for crystallization
Parallel and high throughput protein expression and purification
Crystallization screening and optimization
High-resolution X-ray data collection using in-house source or synchrotron source
Structure determination and analysis
Protein-ligand co-crystal structure determination
Solution and solid states
NMR services
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