Creative Biostructure

Online Inquiry

Please enter "biostructure" as the verification code.

DEBP Protein Crystal

(CBCRY22)  Download Datasheet
Background
Background
The crystal structure of a periplasmic l-aspartate/l-glutamate binding protein (DEBP) from Shigella flexneri complexed with an l-glutamate molecule has been determined and refined to an atomic resolution of 1.0 A. There are two DEBP molecules in the asymmetric unit. The refined model contains 4462 non-hydrogen protein atoms, 730 water molecules, 2 bound glutamate molecules, and 2 Tris molecules from the buffer used in crystallization. The final R(cryst) and R(free) factors are 13.61% and 16.89%, respectively.
Molecular Description
Protein Classification
Transport protein
Structure Weight
64851.95 Da
Polymer
1
Molecule
Periplasmic Binding Transport Protein
Chain Length
287 amino acids
Crystal Description
PDB ID
2VHA
MMDB ID
70491
Source
E.coli
Method
X-Ray Diffraction
Resolution
2.8Å
Ligand Chemical Component
Glutamic acid; 2-amino-2-hydroxymethyl-propane-1,3-diol
Gene Information
Gene Name
livJ
Synonyms
S4285
Gene ID
1080493
Function
Transport activity
Reference
Reference
Hu, Y.L., Fan, C.-P., Fu, G.S., Zhu, D.Y., Jin, Q., Wang, D.-C. (2008) Crystal Structure of a Glutamate/Aspartate Binding Protein Complexed with a Glutamate Molecule: Structural Basis of Ligand Specificity at Atomic Resolution. J.Mol.Biol. 382: 99-111