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MAD2 with p31 Protein Crystal

(CBCRY03)  Download Datasheet
Background
Background
The crystal structure of the Mad2-p31(comet) complex is reported. The C-terminal region of Mad2 that undergoes rearrangement in different Mad2 conformers is a major structural determinant for p31(comet) binding, explaining the specificity of p31(comet) toward Mad1- or Cdc20-bound Mad2. p31(comet) adopts a fold strikingly similar to that of Mad2 and binds at the dimerization interface of Mad2. Thus, p31(comet) exploits the two-state behavior of Mad2 to block its activation by acting as an "anti-Mad2."
Molecular Description
Protein Classification
cell cycle
Structure Weight
105471.24 Da
Polymer
1
Molecule
Mitotic spindle assembly checkpoint protein MAD2A
Chain Length
210 amino acids
Polymer
2
Molecule
MAD2L1-binding protein
Chain Length
268 amino acids
Polymer
3
Molecule
peptide
Chain Length
12 amino acids
Crystal Description
PDB ID
2QYF
Source
E.coli
Method
X-Ray Diffraction
Resolution
2.30 Å
Gene Information
Gene Name
MAD2L1
Synonyms
HSMAD2; MAD2; MAD2 (mitotic arrest deficient, yeast, homolog)-like 1; MAD2-like 1; MAD2-like protein 1; mitotic arrest deficient, yeast, homolog-like 1
UniProt ID
Q13257
Gene ID
4085
Chromosome Location
4q27
Function
protein binding
Gene Name
MAD2L1BP
Synonyms
MAD2L1 binding protein; CMT2, KIAA0110, MGC11282, RP1-261G23.6; OTTHUMP00000016496; caught by MAD2 protein; KIAA0110; dJ261G23.1
UniProt ID
Q15013
Gene ID
9587
Chromosome Location
6p21.1
Function
protein binding
Reference
Reference
Yang, M., Li, B., Tomchick, D.R., Machius, M., Rizo, J., Yu, H., Luo, X. p31comet blocks Mad2 activation through structural mimicry.Cell(Cambridge,Mass.)2007; 131: 744-755