Creative Biostructure

Online Inquiry

Please enter "biostructure" as the verification code.

WDR5 Protein Crystal

(CBCRY09)  Download Datasheet
Background
Background
The WD40 repeat protein WDR5 specifically associates with the K4-methylated histone H3 in human cells. The structure of WDR5 in complex with a dimethylated H3-K4 peptide was deterimined. Unlike the chromodomain that recognizes the methylated H3-K4 through a hydrophobic cage, the specificity of WDR5 for methylated H3-K4 is conferred by the nonconventional hydrogen bonds between the two zeta-methyl groups of the dimethylated Lys4 and the carboxylate oxygen of Glu322 in WDR5. The three amino acids Ala-Arg-Thr preceding Lys4 form most of the specific contacts with WDR5, with Ala1 forming intermolecular hydrogen bonds and salt bridges, and the side chain of Arg2 inserting into the central channel of WDR5.
Molecular Description
Protein Classification
Structural protein and DNA binding protein
Structure Weight
35306.44 Da
Polymer
1
Molecule
WD-repeat protein 5
Chain Length
311 amino acids
Polymer
2
Molecule
Histone H3
Chain Length
18 amino acids
Crystal Description
PDB ID
2G9A
MMDB ID
41532
Source
E.coli
Method
X-Ray Diffraction
Resolution
2.7 Å
Gene Information
Gene Name
WDR5
Synonyms
BIG-3; BIG3; BMP2-induced 3-kb gene protein; SWD3, Set1c WD40 repeat protein, homolog; WD-repeat protein 5; WD repeat domain 5
UniProt ID
P61964
Gene ID
11091
Chromosome Location
9q34
Function
protein binding
Gene Name
WDR5
Synonyms
BIG-3; BIG3; BMP2-induced 3-kb gene protein; SWD3, Set1c WD40 repeat protein, homolog; WD-repeat protein 5; WD repeat domain 5
UniProt ID
P61964
Gene ID
11091
Chromosome Location
9q34
Function
protein binding
Reference
Reference
Han Z, Guo L, Wang H, Shen Y, Deng XW, Chai J. Structural basis for the specific recognition of methylated histone H3 lysine 4 by the WD-40 protein WDR5. Mol. Cell 2006, 22(1):137-44.