Creative Biostructure

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MemPro™ Membrane Protein Families

Creative Biostructure provides one-stop MemPro™ gene-to-structure services for membrane protein production, purification, crystallization, structure determination and characterization. Scientists from Creative Biostructure have rich experience and advanced techniques (e.g., energy-dispersive X-ray spectroscopy, NMR spectroscopy and Cyro-Electron Microscopy) to serve customers with membrane protein structure determination and analysis.

Over 50% of modern medical drug targets at membrane proteins, whose structures and functions are largely unknown. However, membrane proteins are known to be difficult to crystallize with traditional methods because of the rich hydrophobic and insoluble features. Creative Biostructure takes advantage of the MemPro™ cell-free system and high-throughput screening detergents for the optimization of high-yield production and purification strategies for membrane proteins in natural conformations.

Creative Biostructure guarantees the following high-standard services:

√ Harvest of purified and functional membrane proteins with proper documentation;

√ Structural and functional analysis of membrane proteins;

√ Building of structure models for Computer-Aided Drug Design (CADD) and ligand design.

Creative Biostructure can provide custom MemPro™ protein production and analysis services for membrane proteins including but not limited to:

Family name Description
GPCR 7-transmembrane domain receptors transducing extracellular signals into cells.
Ion channels
Transporters
Rhodopsin-like receptors and pumps The most famous GPCRs
ABC transporters Using ATP to translocate various substrates across membranes
Major Intrinsic Protein (MIP)/FNT superfamily Specific for the transport of water and small neutral solutes
Pentameric ligand-gated ion channels Ligand-dependent signal conversion from chemical signals to electric signals.
Ammonia and urea transporters Regulating transepithelial ammonia secretion
Major Facilitator Superfamily (MFS) Facilitating small solute movement in response to chemical concentration gradients of sugars, drugs, etc.
Sulfatase Hydrolysis of sulfate esters; involved in sulfur cycling
Peptidase AD Cleaving amyloid precursor; its dysfunction will cause Alzheimer’s disease.
MAPEG Eicosanoid and Glutathione metabolism proteins
Transmembrane proteins 14 Heme biosynthesis
Hypoxia induced protein Catalyzing the reduction of oxygen; assemble of respiratory
Peptidase family M48 Proteolytical removal of farnesylated C-terminal
Fatty acid desaturase/hydroxylase Biosynthesis of membrane phospholipids, cholesterol ester and triglycerides
Receptor-type kinases Tyrosine-protein kinases, regulate central nervous system, gene transcription and cell differentiation.
T-cell surface glycoprotein CD3 zeta chain Expression of TCR complex.
Immunoglobulin superfamily Recognition, binding and adhesion process of cells. 
Integrin Bridges for cell-cell and cell-extracellular matrix interaction
Toll-like receptors Innate immune system
Protein jagged Notch signaling; embryonic development
TYROBP Containing ITAM; active signal transduction associated with TCR
Stannin Initiatingthe apoptotic cascade
Glycophorin Sialoglycoprotein of the membrane of red blood cell carrying sugar molecules.
SNARE complex Vesicle fusion
BNip3 protein family Protect cell from apoptosis
Bcl-2 Regulating cell-death, either induce apoptotic or inhibit apoptosis.
Single-helix ATPase regulators ATPase regulators; P-P-bond hydrolysis-driven transporter
Voltage-gated K+ channel accessory protein (MinK) Returning the depolarized cell to rest state.
Cytochrome P450 Terminal oxidase enzyme in electron transfer chain.
Transmembrane proteins with NAD(P)-binding Rossmann-fold domains Monoamine oxidase; deaminates norepinephrine, epinephrine, serotonin and dopamine.
Oligosaccaryltransferase Transferring a 14-sugar oligosaccharide from dolichol tonascent protein
N-(deoxy)ribosyltransferase-like superfamily CD38; synthesis and hydrolysis of cyclic ADP-ribose
Peptidase MA Zinc metalloenzymes; Cleave amino acids from the N-terminal
TPR alpha-helical repeat proteins Containing anaphase-promoting complex; mediating protein-protein interaction.
Acyl CoA binding protein Binding with medium/long-chain acyl-CoA esters and functioning as an intracellular carrier of acyl-CoA esters
Amyloid beta A4 protein Involved in alzhemier’s diseases
Lysosome-associated membrane glycoprotein Specific to lysosomes; CD107
Tumor necrosis factor receptor Binding with TNF and NGF; interacting with a variety of signal molecules. Highly associated with apoptosis.
Type I phosphodiesterase/nucleotide pyrophosphatase Hydrolyzing nucleoside 5' triphosphates to their monophosphates and diadenosine polyphosphates.
Squalene/phytoene synthase Cholesterol biosynthetic pathway
FMN-linked oxidoreductases DHODH; biosynthesis of orotate
Neutral/alkaline ceramidase Converting sphingolipid to sphingosine
Aldehyde dehydrogenase ALDH; Oxidation of aldehydes
Mitochondrial and plastid porins Calcium ion transporter in mitochondrial. Regulate cell metabolism and apoptosis
HSD-11B Converting cortisone to cortisol
Family name Description
stearoyl -CoA desaturase 1 A δ-9 fatty acid desaturase that catalyzes the synthesis of monounsaturated fatty acids.
CAAX protease Integral membrane protease located at the endoplasmic reticulum to catalyze proteins contain a CAAX motifs.
EPHA1 A receptor tyrosine kinase that binds membrane-associated ephrin-A family ligands.
EPHA2 A receptor tyrosine kinases that binds membrane-associated ephrin-A family ligands.
EPHA3 A receptor tyrosine kinases that binds membrane-associated ephrin-A family ligands.
EPHA4 A receptor tyrosine kinases that binds membrane-associated ephrin-A family ligands.
HER2/ErbB2 A transmembrane glycoprotein receptor tyrosine kinase involved in the signal transduction pathways.
HER3/ErbB3 A member of the epidermal growth factor receptor (EGFR/ERBB) family of receptor tyrosine kinases; can form active heterodimers with HER2/ErbB2.
HER4/ErbB4 A ubiquitously expressed member of the epidermal growth factor (EGF/ErbB) family of receptor tyrosine kinases.
5-lipoxygenase–activating protein A protein that in humans is encoded by the ALOX5AP gene; necessary for the activation of 5-lipoxygenase and the production of leukotrienes.
Gamma secretase A multi-subunit protease complex that cleaves single-pass transmembrane proteins.
Glucose transporter A group of membrane proteins that facilitate the transport of glucose over a plasma membrane.
Insulin receptor A transmembrane receptor that is activated by insulin, IGF-I, IGF-II and belongs to the group of tyrosine kinase receptors.
LRR-containing membrane proteins A structural motif of 20-30 amino acid stretches that are abundant in the hydrophobic leucine residue.
Leukotriene C4 synthase A enzyme that in humans is encoded by the LTC4S gene; converts leukotriene A4 and glutathione to leukotriene C4.
Microsomal prostaglandin E synthase A member of membrane associated proteins in eicosanoid and glutathione metabolism (MAPE) superfamily that catalyze the biosynthesis of active PGE2 from cyclooxygenases (COX-1- and COX-2) derived PGH2.
VEGF receptors A class of receptors for  vascular endothelial growth factor (VEGF); can be classified as membrane-bound VEGFR (mbVEGFR) and soluble VEGFR (sVEGFR).