Zebrafish (Danio rerio) possesses the physiology complexity of mammalian animals and is one of the mostcommon vertebrate model organisms. Creative Biostructure establishes and provides the MagHelix™ Zebrafish Screening Platforms for scientific and medical research. Zebrafish can be used to study vertebrate development and gene function. On the other hand, it is also an excellent choice to model various diseases and screen novel drugs for medical and therapeutic research.
Figure 1. Phenotype based chemical screening in zebrafish (Cell Commun Signal. 2010)
Zebrafish larvae have been used increasingly within the last decade to assess the effects of chemical compounds. This is an ideal platform for in vivo compound testing due to its several specific features. Their small size makes their embryos and larvae dispensed in microtiter plates. Zebrafish has high fecundity so it is easy to obtain large sample numbers and it is transparent to facilitate noninvasive imaging. Moreover, they have permeability to small molecules which allows drug to administrate by immersion. There are four classes of zebrafish chemical screening assays: morphological screens (to screen chemical chemical genetics and toxicology), behavioral screens (ex. using swimming kinetics to quantify the effects of single compounds), fixed time-point/labeling assays (to obtain information on a cellular or tissue level) and fluorescence assays (reporter-based quantifications). There are various assays based on MagHelix™ Zebrafish Screening Platforms shown in the following table. Take cardiovascular toxicity test as an example, which is a key limiting factor in drug discovery. The one of the features that the zebrafish heart can be dissected rapidly and maintained ex vivo for several days makes zebrafish an excellent model for assessment of cardiovascular toxicity.
MagHelix™ Zebrafish Screening Platforms
Jonathan R mathias, et al. Advances in zebrafish chemical screening technologies. Future Med Chem. 2012 Sep;4(14):1811-22.
Kerrie L Taylor, et al. Small molecule screening in zebrafish: an in vivo approach to identifying new chemical tools and drug leads. Cell Commun Signal. 2010 Jun 12;8:11.
Satish Srinivas Kitambi, et al. Small molecule screening platform for assessment of cardiovascular toxicity on adult zebrafish heart. BMC Physiol. 2012 Mar 26;12:3.