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Mempro™ Detergent-Free Amyloid Beta-sheet Peptides Production

As an undisputed global leader in membrane protein production and manufacture, scientists from Creative Biostructure can offer customized Mempro™ Amyloid beta-sheet peptides production services using detergent-free expression system.

Amyloid beta-sheet peptides is a superfamily which consists of two families: Beta-sheet peptides of amyloid beta A4 protein and Beta-sheet peptides of major prion protein. Amyloid beta A4 protein, also known as APP or A4, belongs to Beta-sheet peptides of amyloid beta A4 protein family. This protein is composed of a large N-terminal extracellular region including heparin-binding and copper-binding sites, a short C-terminal intracellular domain and finally a short hydrophobic transmembrane domain. The N-terminal region of Amyloid beta A4 protein has an alike structure with cysteine-rich growth factors, while it probably serves as a cell surface receptor, which is conducive to neuronal adhesion, axonogenesis, neurite growth and cell mobility. Amyloid-beta40 (Abeta40) and amyloid-beta42 (Abeta42), two main isoforms of Amyloid beta A4 protein have been discovered in the extracellular brain deposits which are related to Alzheimer's disease (AD).

Mempro™ Detergent-Free Amyloid Beta-sheet Peptides Production Figure 1. The structure of Amyloid beta A4 protein, peptide 672-711.
Creative Biostructure has rich professional experience in high quality Amyloid beta-sheet peptides production using detergent-free membrane protein expression system, we can perform various strategies for Mempro™ detergent-free protein production, including:

  • Mempro™ Amyloid Beta-sheet Peptides Production Using Nanodiscs

Nanodisc is self-assembled soluble discoidal phospholipids bilayers, thus a functional stabilized membrane disk can be supplied by Nanodisc for the combination of membrane associated and membrane-bound molecules.

  • Mempro™ Amyloid Beta-sheet Peptides Production Using Amphipols

Amphipols are a new class of surfactants, they are synthesized by forming amide bonds between octylamine and, sometimes sopropylamine. Amphipols are highly usable tools for handling membrane proteins in detergent-free water solutions and generally used for biophysical researches.

  • Mempro™ Amyloid Beta-sheet Peptides Production Using Poly (styrene-co-maleic acid) Lipid Particles (SMALPs)

SMALPs are disc like structures including a protein in a lipid bilayer, parceled by the amphipathic styrene maleic acid polymer (SMA). As a biochemical approach, SMALP has a significant potential, for it makes membrane bilayers solubilized and it can implant discs in detergents-free condition.

These novel detergent-free approaches for Amyloid beta-sheet peptides production can be obtained easily, and enabling more comprehensively structural and functional studies.

Creative Biostructure provides other various Mempro™ membrane protein production services. Please feel free to contact us for a detailed quote.

References:
Roch, J. M. (1994). “Increase of synaptic density and memory retention by a peptide representing the trophic domain of the amyloid beta/A4 protein precursor”. Proceedings of the National Academy of Sciences 91(16), 7450-7454.
Buxbaum, J. D. (1993). “Protein phosphorylation inhibits production of Alzheimer amyloid beta/A4 peptide”. Proceedings of the National Academy of Sciences 90(19), 9195-9198.
Kubota, T. (2014). “Amyloid beta (A4) precursor protein expression in human periodontitis-affected gingival tissues”. Archives of oral biology 59(6), 586-594.
OPM, The structure of Amyloid beta A4 protein, peptide 672-711. November 2016 http://opm.phar.umich.edu/protein.php?pdbid=2lnq


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