Mempro™ Cell-Based Integrin Production

Creative Biostructure provides custom Mempro™ integrin production services using cell-based expression system.

Mempro™ cell-based protein production system is widely used for the production of integral membrane proteins. Integrins are a kind of transmembrane receptors that are involved in cell adhesion, thus, which are also called cell adhesion receptors. It is reported that integrins play a crucial role in the lower affinity attachment between cells and extracellular matrix (ECM) or various ligands on other cell surfaces. Intergrins are noncovalent-linkage transmembrane αβ heterodimers, containing more than 18 α and 8 β subunit types in human capable of forming 24 different heterodimers. Integrins have been identified in a large variety of species, including mammals, chicken, zebrafish, Caenorhabditis elegans and Drosophila melanogaster.

Figure 1. Integrin-mediated the modulation of actin dynamics. (EMBO J., 2003)

Creative Biostructure can produce high-quality integrins with cell-based protein expression system, we can perform various strategies for Mempro™ cell-based protein production, including:

• Mempro™ Protein Production in Bacterial Cells System ;
• Mempro™ Protein Production in Yeast Cells System;
• Mempro™ Protein Production in Incest Cells System;
• Mempro™ Protein Production in Mammalian Cells System.

Escherichia coli (E. coli) is the most commonly employed bacterial host for the production of integrins. Yeast cells (such as saccharomyces cerevisiae and pichia pastoris) system, combines prokaryotic as well as eukaryotic characteristics. It is a powerful eukaryotic host due to single cells, fast growth rates, inexpensive media, high cell densities. Insect cells (such as Sf9, Sf21 and High Five) are also the major system for integrin expression, which are developed from transfected insect cells in combination with vectors derived from the baculovirus species AcMNPV. Mammalian cells present another eukaryotic system for integrin expression, which provide near-native cell environment. Cell lines derived from COS, CHO, BHK-21, HEK293, HeLa and GH3 can be widely used for transmembrane protein expression.

With the Mempro™ cell-based protein production platform, Creative Biostructure is capable of expressing, isolating, purifying and crystallizing integrins to facilitate the study of their biological functions.

We provide other various Mempro™ membrane protein production services. Please feel free to contact us for a detailed quote.

References:
A. van der Flier and A. Sonnenberg (2001). Function and interactions of integrins. Cell Tissue Res., 305(3): 285-298.
C. Brakebusch and R. Fässler. (2003). The integrin-actin connection, an eternal love affair. EMBO J., 22(10):2324-2333.
C. Trometer, and P. Falson (2010). Mammalian membrane protein expression in baculovirus-infected insect cells. Methods Mol. Biol., 601: 105-117.
F. Junge, et al. (2008) Large-scale production of functional membrane proteins. Cellular Mol. Life Sci., 65 (11): 1729-1755.
J. Petschnigg, et al. (2011). Using yeast as a model to study membrane proteins. Curr. Opin. Nephrol. Hypertens., 20(4): 425-432.