Mempro™ Cell-Free Lipocalin Production

Creative Biostructure can provide custom Mempro™ protein production services for lipocalins using cell-free expression system.

Mempro™ cell-free protein production system is the progressive platform to produce intergral membrane proteins which can bypass the limitations that encountered in cell-based protein production system, such as host cell toxicity, low yield and solubilization and purification using detergents.

Members of lipocalin protein family, which contain a buried structural pocket as a binding site, can form a different group of ligand-binding proteins that play important roles in transprot, storage, or sequestration of a variety of small hydrophobic ligands. Most lipocalins possess a signal peptide at N-terminus, and several disulfide bridges that contribute to constraint of the overall structure through keep sabilization of the N- and C-terminal regions of the lipocalin. It has been reported that lipocalins are associated with a variety of biological processes, such as cancer cell interactions, biological prostaglandin synthesis, immune response, pheromone transport and retinoid binding.

Figure 1. The structure of human lipocalin. (Acc. Chem., 2015)

Creative Biostructure has extensive experience in high-yield lipocalin using cell-free protein expression system, we can provide various strategies for Mempro™ cell-free protein production with post-tranlational modifications, including:

• Mempro™ Cell-Free Protein Production in E. coli;
• Mempro™ Cell-Free Protein Production in Wheat Germ;
• Mempro™ Cell-Free Protein Production in Rabbit Reticulocyte;
• Mempro™ Cell-Free Protein Production in Baculovirus.

Among Mempro™ cell-free protein production platform, E. coli S30 extract is popular used for lipocalin production. Wheat germ extract can provide eukaryotic translation system for the production of lipocalins. Baculovirus-based expression system derived from Autographa californica nucleopolyhedrovirus (AcNPV) is also a major system for lipocalin expression. Notably, crude rabbit reticulocyte lysate is the most important tool used for eukaryotic cell-free membrane protein expression system.

With the Mempro™ cell-free protein production platform, Creative Biostructure can express, purify, solubilize and crystallize lipocalins to study interactions with other proteins for molecular recognition. Mempro™ cell-free protein production has remarkable advantages, including：

• Very high yield;
• Low cost;
• Lack of toxicity;
• Incorporation of non-natural amino acids into protein structure;
• Relatively tolerant of additives.

Creative Biostructure can provide various custom Mempro™ membrane protein production services. Please feel free to contact us for more information.

References:
A. Schiefner and A. Skerra (2015). The Menagerie of Human Lipocalins: A Natural Protein Scaffold for Molecular Recognition of Physiological Compounds. Acc. Chem., 48(4): 976–985.
Lipocalin. (https://en.wikipedia.org/wiki/Lipocalin)
M. D. Ganfornina, et al. (1999). A phylogenetic analysis of the lipocalin protein family. Mol. Biol. Evol., 17(1): 114-126.