The List of Kinases at Creative BioStructure

Creative BioStructure has extensive experience in co-crystallization of a large number of protein kinases with small molecule compounds. Below please see the list of kinases we have been working on.

• Since most protein kinases of public interest have their structures resolved or have a homologous structure available, it is much easier to express a kinase in a soluble functional form [or refold it into such a form] in comparison with novel proteins.

• Also, we may follow [or refer to] the published conditions to grow the crystals, the most challenging step in X-ray Crystallography.

• Most importantly, if structure of a homologous kinase is available in the RCSB Protein Data Bank (http://www.rcsb.org/pdb/home/home.do), the kinase-compound structure can be determined by one good set of diffraction data with Molecular Replacement (MR) method.

• In contrast, if a homologous model is not available, phase problem has to be determined by either Multiple Isomorphous Replacement (MIR) using heavy atoms or Multiple Wavelength Anomalous Dispersion (MAD) using selenomethionine derivatives. Depending on the phasing methods, more than one data set is collected.