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Plasmids Design and Construction

The Plasmid Design and Construction platform developed by Creative Biostructure is aimed to rapidly and reliably deliver application-specialized plasmids. We are specialized in the construction of custom plasmids, starting from initial design strategy to the plasmids construction process. Before you start any project, Creative Biostructure also provides protein domain prediction services based on bioinformatics and biochemical analysis.

Plasmids-Powerful Biotechnology Tools

Plasmids are essentially tiny, self-contained genomes that replicate independently, and generally do not have adverse effects on the host cells. Therefore, they are extraordinarily useful tools to manipulate genes.

Protein Domain Prediction

In most cases, proteins contain more than one structural or functional domain. The fold and function of protein domains can be evolved independently. It is with great importance to perform an accurate prediction of the location of these domains for sequence analysis, similarity searching, protein production, and protein function investigation.

Plasmid Design and Construction Figure 1. Domain annotation and prediction of the protein models by using the ORF2 protein as an example, further quality determination was performed as well. G. Abrusan, et al., 2013)

The most accurate analysis of protein domains is based on the 3D structures of proteins. However, the experimental determination methods are usually time-consuming and expensive. Furthermore, it is quite difficult to obtain 3D structures of some proteins, especially for membrane proteins. Therefore, Creative Biostructure highly recommend our Protein Domain Prediction platform based on amino acid sequence analysis.

We can predict secondary structure and returning families of your target proteins, analyze the solvent accessibility, and predict transmembrane regions. We have further expanded the breadth of our protein domain prediction services by adding analysis of non-regular secondary structure, intrinsically disordered regions, inter-residue contacts, disulfide bridges, and transmembrane beta-barrel structures. As to protein function prediction, we are able to assist in annotation of subcellular localization and identifying protein binding sites or protein-protein interaction sites.

Plasmids Construction

Creative Biostructure is proud to offer a wide variety of custom plasmid vector construction services including constitutive and inducible vectors for various expression systems (bacterial, yeast, mammalian, insect). We can also assist you in case your required plasmid vector is not available. Creative Biostructure has developed an advanced gene synthesis platform to modular construct plasmid vectors. There are individual modules with easily exchangeable fragments, which encode not only regulatory and functional elements but also custom-made elements. We obtain these modules by PCR amplification or synthetical methods to fit your unique requirements. Creative Biostructure is willing to help you develop completely customized vectors by using complex cloning strategies, and we promise that you can retain any intellectual properties related to the resulting plasmid vectors. Our plasmid construction services include sequences analysis, vector design, gene synthesis, PCR cloning, DNA fragment assembling, DNA sequencing, site directed mutagenesis, and plasmid production, etc.

Plasmid Design and Construction Figure 2. Schematic representation of targeting vector construction. (H. M. Prosser, et al., 2011)

Besides Plasmid Design and Construction services, a variety of products and services  related to protein production and expression are available as well. Creative Biostructure is your trustworthy and reliable business associate. Please feel free to contact us for a detailed quote.

References:

H. M. Prosser, et al. (2011). A resource of vectors and ES cells for targeted deletion of microRNAs in mice. Nature Biotechnology, 29: 840–845.

S. M. Yoo, et al. (2013). Design and use of synthetic regulatory small RNAs to control gene expression in Escherichia coli. Nature Protocols,8: 1694–1707.

J. W. Bok, et al. (2012). Fast and easy method for construction of plasmid vectors using modified Quick-change mutagenesis. Methods Mol. Biol., 944:163-174.

K. Drew. (2011). The proteome folding project: Proteome-scale prediction of structure and function. Genome Research, 21(11): 1981–1994.

G. Abrusan, et al. (2013). Structure Prediction and Analysis of DNA Transposon and LINE Retrotransposon Proteins. Journal of Biological Chemistry, 288: 16127-16138.


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