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Plasmids Design and Construction

Plasmid vector construction is an essential step in molecular biology. The plasmid design and construction platform developed by Creative Biostructure is aimed to deliver application-specialized plasmids design and construction services. Our services include constitutive and inducible vectors for various expression systems. We can also assist you in case your required plasmid vector is not available.

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Following is a list of vectors available in different expression systems:

Expression Vectors for Bacterial Systems
pGS-21a pETDuet-1 pCDFDuet-1 pCOLADuet-1 pET-3a pET-3b
pET-3c pET-3d pET-9a pET-11a pET-11b pET-11c
pET-11d pET-14b pET-15b pET-16a pET-17b pET-19b
pET-20b(+) pET-21a(+) pET-21b(+) pET-20d(+) pET-22b(+) pET-23a(+)
pET-24a(+) pET-24b(+) pET-24c(+) pET-24d(+) pET-25b(+) pET-26b(+)
pET-27b(+) pET-28a(+) pET-28b(+) pET-28c(+) pET-29a(+) pET-29b(+)
pET-29c(+) pET-30a(+) pET-30b(+) pET-30c(+) pET-31b(+) pET-32a(+)
pET-32b(+) pET-41a(+) pET-41b(+) pET-41c(+) pET-42a(+) pET-42b(+)
pET-42c(+) pET-43.1a(+) pET-43.1b(+) pET-45b(+) pET-50b(+) pET-51b(+)
pET-52b(+) pGEX-2TK pGEX-4T-1 pGEX-4T-2 pGEX-4T-3 pGEX-5X-1
pGEX-5X-2 pGEX-5X-3 pGEX-6P-1 pGEX-6P-2 pGEX-6P-3 pMAL-c4x
pMAL-c5E pMAL-c5x pMAL-p5E pMAL-p5G pMAL-p5X pQE-1
Expression Vectors for Yeast Systems
pPIC3.5k pPIC9 pPIC9K pPICZA pPICZB pPICZC
pPICZakohaA pPICZakohaB pPICZakohaC pESC-TRP pESC-URA pESC-HIS
Expression Vectors for Insect Systems
pBacPAK8 pBacPAK9 pAcG2T pAcHLTA pAcHLTB pAcHLTC
pAcGHLTA pAcGHLTB pAcGHLTC pAcSG2 pBAC-1 pFastBac1

Expression Vectors for Mammalian Systems
pcDNA3.1(+) pcDNA3.1(-) pcDNA3.1(+)_myc-HisA pcDNA3.1(+)_myc-HisB
pCI-Neo pcDNA3.1/Zeo(+) pcDNA3.1(+)_myc-HisC pcDNA3.1(-)_myc-HisA
pcDNA3.1/Zeo(-) pCMV-3Tag-1a pcDNA3.1(-)_myc-HisB pcDNA3.1(-)_myc-HisC
pCMV-3Tag-2a pCMV-3Tag-3a pcDNA3.1+C-DYK pcDNA3.1+C-HA
pCMV-3Tag-4a pcDNA3.1+C-eGFP pcDNA3.1+C-6His pcDNA3.1+C-Myc
pcDNA3.1+P2A-eGFP pcDNA3.1+N-eGFP pcDNA3.1+N-DYK pcDNA3.1+N-HA
pcDNA3.1+P2A pET-42(+) pcDNA3.1+N-6His pcDNA3.1+N-Myc
pET-43.1a(+) pET-43.1b(+) pcDNA3.1+N-GST(TEV) pcDNA3.1/Hygro(+)
pET-45b(+) pET-52b(+) pcDNA3.1/Hygro(-) pcDNA3.1+C-DYK-P2A
pGEX-2TK pGEX-4T-1 pcDNA3.1+N-DYK-P2A pGEX-4T-1-M(RBS)
pGEX-4T-2 pGEX-5X-2 pGEX-5X-1-H(RBS) pGEX-5X-1-M(RBS)
pGEX-5X-3 pGEX-6P-1 pGEX-6P-1-H(RBS) pQE-60
pGEX-6P-2 pMAL-c5E pGS-21a pETDuet-1
pMAL-c5x pMAL-p5E pMAL-p5G pCDFDuet-1

Plasmids Design and Construction

Creative Biostructure performs the custom plasmid construction from design to production. We have successfully designed and constructed numerous plasmid vectors. With our extensive experiences, we are certain that we can help with your research projects, please feel free to contact us for a detailed quote

Ordering Process

Ordering Process

Reference:

  1. S. M. Yoo, et al. (2013). Design and use of synthetic regulatory small RNAs to control gene expression in Escherichia coli. Nature Protocols, 8: 1694–1707
  2. J. W. Bok, et al. (2012). Fast and easy method for construction of plasmid vectors using modified Quickchange mutagenesis. Methods Mol. Biol., 944:163-174
  3. S. Kwon, et al. (2015). Comparative genomics and experimental evolution of Escherichia coli BL21(DE3) strains reveal the landscape of toxicity escape from membrane protein overproduction. Scientific Reports, 5:16076

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