Creative Biostructure to Present at AACR Annual Meeting 2024 | April 5-10, 2024 | Booth #2953 
Exosome Analysis Services
Exosomes contain proteins, lipids, nucleic acids, and metabolites, it has become a topic of interest in medical research and molecular biology. With a unique molecular signature and it can be easily enriched from biofluids, which can provide very valuable information for early detection and disease monitoring. The composition and quantity of exosomes may be directly related to the pathological state of the patient, so the analysis of exosomes could be of great relevance for the diagnosis and prognosis of different cancers.
Figure 1. Exosomes in liver metastasis of colorectal cancer. (Biting Zhou, et al. 2020)
Therefore, to acquire a deeper knowledge of exosome biology and accelerate the use of these extracellular vesicles in diagnostic tests or clinical research, Creative Biostructure provides sensitive, reproducible methods for exosome analysis.
Exosome Tracking Service
The exosome tracking service enables real-time monitoring and visualization of the dynamic distribution and functionality of exosomes within living organisms. Leveraging our significant expertise in exosome projects, we support customers in acquiring profound insights into the intricate mechanisms of exosomes in biological processes, offering crucial assistance for their clinical applications.
Exosome RNA Sequencing Services
Nucleic acid is another important cargo that exosomes carry, including deoxynucleic acids (DNA), coding and non-coding ribonucleic acid (RNA) like messenger RNA (mRNA) and microRNA (miRNA). After exosome isolation, nucleic acids need to be accurately quantified in order to track many kinds of medical conditions and diseases, especially when nucleic acids of exosomes are greatly different from the parental cell content.
Creative Biostructure provides a complete exosome workflow solution to solve nucleic acids profiles of exosomes, starting from fast and efficient isolation of RNA to exosomal RNA sequencing using qRT-PCR and novel sequencing techniques.
Exosome Lipidomics & Metabolomics Services
Lipids, an important part of cellular physiology, are the major components of exosomes. The importance of lipids in exosome biology has been associated with the fact that the lipid content of exosomes differs from the parent cells and changes during physiological processes. Exosome lipidomics study provides great access to identify lipid-based biomarkers and unravel its monitoring function in diseases. Besides, a wide range of metabolites in exosomes could also be used for biomarker discovery by showing distinct metabolite populations during different disease treatment stages.
Our customized services provide comprehensive support for exosomal lipidomics & metabolomics study by using different analysis methods for data acquisition, analysis, and even interpretation: Thin layer chromatography (TLC), High-performance liquid chromatography (HPLC), NMR spectroscopy, Mass spectrometry, etc.
Exosome Proteomics Services
Exosome proteomics has been recognized as easy access for biomarkers monitoring, providing information on the physiology study of the parent cells and exosomes. For analysis by mass spectrometry, exosomal proteins can be separated by SDS-PAGE, in-gel digested, followed by LC-MS/MS experiments. Based on our customer need, we can work from exosome isolation to data collection and analysis, a final report with results will be provided.
Nano-Flow Cytometry (nFCM) Service
Nano-flow cytometry (nFCM) technology is an emerging method for nanoparticle characterization, which can detect exosomes with sizes as low as 30 nm. It can provide high-resolution size distribution comparable to TEM, determine particle concentration of whole population as well as subpopulation, and quantify multiple parameters simultaneously.
Creative Biostructure's exosome analysis services make it easy to profile exosome lipids, proteins and RNAs with our years of experience working on exosomes. Our expertise provides comprehensive support for your exosome study.
Ordering Process
References
- Biting Zhou et al. Application of exosomes as liquid biopsy in clinical diagnosis. Signal Transduct Target Ther. 2020, 5: 144.
- Sitar Simona et al. Size characterization and quantification of exosomes by asymmetrical-flow field-flow fractionation. Anal Chem. 2015, 87(18): 9225-9233.
- A comprehensive view of the characterization of exosomes. Sponsored by Wyatt Technology. 2018.
- Tatiana Altadill et al. Enabling metabolomics based biomarker discovery studies using molecular phenotyping of exosome-like vesicles. PLoS One. 2016, 11(3): e0151339.
- Alicia Llorente et al. Molecular lipidomics of exosomes released by PC-3 prostate cancer cells. Biochim Biophys Acta. 2013, 1831(7): 1302-1309.
- Kevin L Schey et al. Proteomics characterization of exosome cargo. Methods. 2015, 87: 75-82.
- Jeoffrey Schageman et al. The complete exosome workflow solution: from isolation to characterization of RNA cargo. Biomed Res Int. 2013, 2013: 253957.
