Liposomes are artificial spherical vesicles with enclosed phospholipid bilayer structures. They are most often composed of phospholipids, especially phosphatidylcholine, but may also include other lipids such as egg phosphatidylethanolamine, as well as pure surfactant components like DOPE (dioleoylphosphatidylethanolamine). Liposomes are now an established technology platform with considerable clinical acceptance for the delivery of anti-cancer, anti-fungal, anti-inflammatory and antibiotic drugs, as well as nucleic acid-based therapeutics. Besides, liposomes are also frequently used as supplements to assist membrane protein synthesis, forming proteoliposomes, which serve as a model for the study of many membrane proteins.
Creative Biostructure has established proteoliposomes as a powerful tool to study the function of membrane proteins. With the membrane protein screening platform, our scientists at Creative Biostructure have been optimizing different techniques to insert proteins into liposomes, including mechanical means, freeze-thawing, organic solvents, and detergent-mediated method. The detergent-mediated pathway is most frequently used at Creative Biostructure due to its simplicity and robustness.
Figure 1. Solvation of membrane protein in liposome
Features of our service
Creative Biostructure takes advantage of the most advanced liposome technology to reconstitute membrane proteins in their native state and conformation. This greatly facilitates the structural/functional studies of transmembrane or membrane-associated proteins. Please see the list of membrane proteins provided by Creative Biostructure.
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Claxton DP, et al. (2015) Navigating Membrane Protein Structure, Dynamics, and Energy Landscapes Using Spin Labeling and EPR Spectroscopy. Methods Enzymol 564:349-387.