Creative Biostructure

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MagHelix™ Assay Development

Creative Biostructure provides high-quality custom MagHelix™ assay design and development services for pharmaceutical, biotechnological and agrochemical as well as scientific research and development industries. We can start from a particular target/cell line or assist an ongoing lead discovery programs based on the requirements of our clients.

MagHelix™ Assay DevelopmentFigure 1. Assay development cycle.

In general, the quality of an assay is defined by the robustness and reproducibility of this signal in the absence of any test compounds or in the presence of inactive compounds. The quality of the high-throughput screening (HTS) is then defined by the behavior of this assay system when screened against a series of compounds. Assay development for HTS can be roughly characterized as cell-free or cell-based in nature. The aim of assay development is to transfer the assay parameters to a new format, thereby enhancing dynamic range, sensitivity, signal intensity or stability of the assay. An assay consists of three general steps: First, the identification step discriminates the analyte from the background noise by targeting its specific properties. Second, the amplification step translates the quantity or presence of the discriminated analyte into a detectable signal. At last, the amplified signal is detected and converted into a comprehensible output. During assay development the optimization process usually concentrates on these three steps.

Our experts are capable of developing and performing biochemical/cell-based assays and multi-target profiling assessment for various receptors, enzymes and other proteins. We have many years of experience in transferring, optimizing and validating customized assays to fit our clients’ needs. Utilizing our technical expertise in cell biology, molecular biology, microbiology and analytical chemistry, Creative Biostructure can also work with customers to design and develop novel assays. Our seasoned scientists are able to transform the assays into high throughput screening (HTS) approaches, which are extremely appropriate for in-process analysis, bulk, and final product lot release (e.g., biosafety, purity, potency, identity and concentration). All assay procedures, specifications and results can be precisely documented for our customers.

Our technologies of assays include but are not limited to radioactivity, flow cytometry, electrochemiluminescence, fluorescence, mass spectrometry, surface plasmon resonance, luminescence, alpha screen, scintillation proximity assay and protein chemistry assays (e.g., ELISA, UV-Vis, X-ray & NMR).

A principle for alpha sureFire assay development. Figure 2. A principle for alpha sureFire assay development.

Creative Biostructure supplies the following MagHelix™ in vitro assay services that will benefit your projects:
Assay Development

  • Assay Technologies.

Library Design and Screening

  • Targets
  • Targeted diseases
  • Library screening

Hit to Lead

  • Hit evaluation and lead identification
  • Mode of action
  • X-ray Crystallography
  • Customized NMR Services

ADMET (Absorption, Distribution, Metabolism, Excretion and Toxicity) Modeling and Profilling

Natural Products

Creative Biostructure can provide various custom MagHelix™ drug discovery services. Please feel free to contact us for a detailed information.

References:
Inglese et al., (2005) Guidance for assay development and high-throughput screening. NIH Chemical Genome Center.


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