Mempro™ Cell-Free Peptidase AD Production

Creative Biostructure has developed the comprehensive membrane protein production through years of experience, our experts can produce high-quality peptidases AD using cell-free expression system. Our excellent cell-free expression platform can bypass the limitations that encountered in cell-based protein production system, including host cell toxicity, low yield and solubilization and purification using detergents.

Peptidase AD, also called gamma secretase complex, which plays an important role in the progress of Alzheimer’s disease (AD). Peptidase AD is an 18-spanning transmembrane aspartyl protease that can regulate intramembrane proteolysis for a growing list of integral membrane proteins. The most famous cleavage substrate of peptidase AD is amyloid precursor protein, which is closely related to AD. It has been shown that peptidase AD superfamily is composed of three protein families: Type IV leader peptidase, Presenilin and Gamma-secretase. Gamma-secretase family contains seven members, including Gamma-secretase complex, structure 1; Gamma-secretase complex, structure 2; Gamma-secretase complex, structure 3; Gamma-secretase complex, structure 4; Gamma-secretase complex, structure 5; Gamma-secretase complex, structure 6 and Nicastrin, TM helix. Up to now, several drugs act as gamma-secretase inhibitors have been developed to treat Alzheimer’s diseases.

Figure 1. Structural model of intramembrane aspartate protease. (OPM Database)

Creative Biostructure offers various strategies for high-quality peptidases AD, including:

• Mempro™ Peptidase AD Production in Bacterial Extract
E. coli S30 extract is widely used for membrane protein production. Mempro™ membrane protein expression by bacterial extract provides you the enhancement of expression of desired membrane proteins, and easy to perform scale-up membrane protein production.

• Mempro™ Peptidase AD Production in Wheat Germ
Creative Biostructure provides eukaryotic translation system for Peptidases AD production in wheat germ system. This system facilitates the membrane proteins performing correct folding and post-translational modifications and maintaining the correct structural and functional characteristics.

• Mempro™ Peptidase AD Production in Rabbit Reticulocyte
Crude rabbit reticulocyte lysate is the outstanding system for eukaryotic membrane protein production, providing multiple options for the optimization of the production protocols, such as the availability of materials, protein origin and complexity, downstream processing, etc.

• Mempro™ Peptidase AD Production in Baculovirus
Baculovirus expression system from Autographa californica nucleopolyhedrovirus (AcNPV) is also a powerful system for transmembrane protein expression, which is developed in the early stage that transfected insect cells in combination with vectors derived from the baculovirus.

Creative Biostructure can also provide Mempro™ plant-based virus-like particles (VLPs) production services, Mempro™ animal-free recombinant protein production services and Mempro™ membrane protein production services. Please feel free to contact us for a detailed quote.

References:
F. Bernhard, et al. (2013). Cell-free expression - making a mark. Cur. Opin. Struct. Biol., 23: 374-380.
S. Krishnaswamy, et al. (2009). The structure and function of Alzheimer’s gamma secretase enzyme complex. Critical Rev. Clinical Lab. Sci., 46(5-6): 282-301.