Mempro™ Cell-Free Protein Kinase Superfamily Production

Creative Biostructure has established a comprehensive membrane protein production platform through years of experience. We can provide first-class custom protein kinase superfamily production services through our excellent Mempro™ cell-free protein expression system.

Mempro™ cell-free protein production system is the innovative platform developed by Creative Biostructure, which can bypass the limitations that encountered in cell-based protein production system, such as host cell toxicity, low yield and solubilization and purification using detergents. Protein kinases are a group of kinase enzymes that catalyze the reversible phosphorylation of numerous proteins. With the variation of cellular location, kinase enzyme activity or connection with other proteins, phosphorylation generally leads to a functional change of the target protein. The protein kinase superfamily belongs to one of the largest homologous protein superfamilies, there are approximately 500 protein kinases in the human genome, and they form about 2% of the whole human genes. Nearly 30% of all human proteins possibly can be modified by kinase, moreover, kinases are found to control most of cellular pathways, particularly proteins which are related to signal transduction. Plants and bacteria are able to discover protein kinases as well.

Figure 1. Schematic of protein phosphorylation. (Wikipedia)

Creative Biostructure is knowledgeable in performing various strategies for high-quality cell-free expressed protein kinase superfamily proteins, including:

• Mempro™ Protein Kinase Superfamily Production in Bacterial Extract
E. coli S30 extract is commonly applied for membrane protein production. Mempro™ membrane protein expression by bacterial extract provides you the enhancement of expression of the protein kinases, and easy to perform scale-up membrane protein production.

• Mempro™ Protein Kinase Superfamily Production in Wheat Germ
Creative Biostructure provides eukaryotic translation system for protein kinases production in wheat germ system. This system facilitates the membrane proteins performing correct folding and post-translational modifications and maintaining the correct structural and functional properties.

• Mempro™ Protein Kinase Superfamily Production in Rabbit Reticulocyte
Crude rabbit reticulocyte lysate is the renewed system for eukaryotic membrane protein production, providing multiple options for the optimization of the production protocols, such as the availability of materials, protein origin and complexity, downstream processing, etc.

• Mempro™ Protein Kinase Superfamily Production in Baculovirus
Baculovirus expression system from Autographa californica nucleopolyhedrovirus (AcNPV) is also an powerful host for protein kinases expression, which is developed in the early stage that transfected insect cells in combination with vectors derived from the baculovirus.

Creative Biostructure can also provide Mempro™ plant-based virus-like particles (VLPs) production services, Mempro™ animal-free recombinant protein production services and Mempro™ membrane protein production services. Please feel free to contact us for a detailed quote.

References:
F. Bernhard, et al. (2013). Cell-free expression - making a mark. Cur. Opin. Struct. Biol., 23: 374-380.
G. Manning, et al. (2002). The protein kinase complement of the human genome. Science, 298(5600): 1912-1934.
N. Dhanasekaran, , and E. P. Reddy (1998). Signaling by dual specificity kinases. Oncogene, 17(11), 1447-1455.