Mempro™ Detergent-Free Immunoglobulin Superfamily Protein Production

Based on the comprehensive protein engineering platform established through years of experiences, scientists form Creative Biostructure can provide advanced custom Mempro™ immunoglobulin superfamily (IgSF) protein production services using detergent-free expression system.

Immunoglobulin superfamily (IgSF) is a large group of proteins that play important roles in recognition, binding, or adhesion processes of cells, including CD4, CD8, T cell receptor (TCR), and HLA. Members of IgSF possess a structural domain termed immunoglobulin (Ig) domain, which can be found in numerous proteins, including cell surface antigen receptors, co-receptors and co-stimulatory molecules of the immune system, cell adhesion molecules, cytokine receptors and intracellular muscle proteins. Conventional approaches for membrane protein production require extraction from the membrane into detergent micelles. However, detergent can induce conformational changes on the membrane proteins. Our substitutive detergent-free expression system can best maintain the native conformation of IgSF proteins for further structural analysis.

Figure 1. The structural model of Hepatitis A virus cellular receptor 1 homolog. (OPM database)

Creative Biostructure has extensive expertise in high-yield IgSF protein production based on detergent-free membrane protein expression system, we can perform various strategies for Mempro™ detergent-free IgSF protein production, including:

• Mempro™ IgSF protein production using nanodiscs

  Nanodiscs are the self-assembled nanotechnology to stabilize membrane proteins removed from the membrane by membrane scaffold proteins (MSPs) in absence of detergent. Creative Biostructure can reconstitute IgSF proteins into nanodiscs in detergent-free process: Expressing the IgSF proteins in the cell-free expression system with the addition of pre-assembled nanodiscs.

• Mempro™ IgSF protein production using amphipols

  The amphipathic amphipols have the ability to “trap” around transmembrane proteins, allowing them to stay folded. Creative Biostructure can apply A8-35 to solubilize IgSF proteins during purification and stabilization processes.

• Mempro™ IgSF protein production using poly (styrene-co-maleic acid) lipid particles (SMALPs)

The SMALPs can be self-assembled by the simple addition of the SMA co-polymers. At neutral or alkaline pH, the disc-like structure assembles itself, encapsulating the IgSF proteins in a formation amenable to be purified.

These novel detergent-free techniques for membrane protein production can be obtained easily, and enabling more comprehensively structural and functional studies.

Creative Biostructure provides other various Mempro™ membrane protein production services. Please feel free to contact us for a detailed quote.

References:
C. Tribet, et al. (1996). Amphipols: Polymers that keep membrane proteins soluble in aqueous solutions. Proc Natl Acad Sci U. S. A. 93: 15047-15050.
K. Natarajan, et al. (2015). Immunoglobulin superfamily. eLS, doi: 10.1002/9780470015902.a0000926.pub2
M. Jamshad, et al. (2011). Surfactant-free purification of membrane proteins with intact native membrane environment. Biochem. Soc. Trans., 39: 813-818.
Y. Shimono, et al. (2012). Immunoglobulin superfamily receptors and adherens junctions. Subcell Biochem., 60: 137-170.