Mempro™ Plant-Based Lipoxygenase Production

Mempro™ membrane protein production platform developed by Creative Biostructure can produce high-quality lipoxygenases using plant-derived protein expression system.

It is well known that lipoxygenases are short for LOXs, which are a large found in various organisms, such as planta, animalia, bacteria and fungi. LOX consists of a structural related family of iron-containing enzymes that can catalyze the addition of oxygen to esstial polyunsaturated fatty acids possessing a cis, cis-1, 4- pentadiene structure in human. Their functions include initiating the signaling molecule synthesis and inducing the structural or metabolic changes in the cell. Members of LOX proteins share highly conserved domains and sequence motifs that are responsible for their distinct structure and the interaction with the catalytic iron, for instance the “LOX motif”: His-X4-His-X4-His-X17-His-X8-His. In mammalian cells and plant cells, LOX is composed of two domains: a N-terminal β-barrel domain named PLAT (Polycystin-1, lipoxygenase, Alpha-Toxin) and a C-terminal catalytic domain LH2 (Lipoxygenase homology) containing a single atom of non-heme iron. This iron atom is bound to conserved histidines and to the carboxyl group of a conserved C-terminal isoleucine.

Figure 1. The model of LOXs and other enzymes of the oxylipins pathway in plant cell. (Plant Physiol., 2002)

Creative Biostructure has rich professional knowledge in providing unparalleled membrane protein production services based on various types of plants, such as Nicotiana benthamiana (tobacco), Medicago rativa (alfalfa), Arabidopsis thaliana (A. thaliana), potato, maize, barley and lettuce. We can perform two different strategies for recombinant membrane protein production in plants: transient expression and stable transformation.

Mempro™ membrane protein production platform using plant system has several key features, such as animal-free, low protease activity, and low endotoxin. Animal-free recombinant membrane proteins are particularly crucial for customers concerned with experimental variables caused by trace animal components or mammalian pathogens. Recombinant membrane proteins expressed by our plant system are free of animal components, serum, endotoxins and antibiotics as well as human or animal infectious agents or other endogenous mammalian contamination. In addition, post-translational modification such as glycosylation and disulfide bonds can be introduced in plant system.

Advantages of Mempro™ plant-based membrane production platform:

• Cost-effective
• Easy storage and distribution
• Environment friendly
• Free of infectious or toxic contaminants
• High yield
• Post-translational modifications

With the Mempro™ plant-based protein production platform, Creative Biostructure is capable of expressing, isolating and purifying lipoxygenases to facilitate the study of their structural and biological functions. We can overcome the common difficulties encountered in cell-based expression system, such as misfolding, aggregation, inactivity, high endotoxin, poor stability and solubility, etc.

Creative Biostructure can also provide Mempro™ plant-based virus-like particles (VLPs) production services, Mempro™ animal-free recombinant protein production services and Mempro™ membrane protein production services. Please feel free to contact us for a detailed quote.

References:
A. Wiktorek-Smagur, et al. (2012). Green way of biomedicine – how to force plants to produce new important proteins. Transgenic Plants-Advances and Limitations, Chapter 3. doi: 10.5772/1409.
H. Porta and M. Rocha-Sosa (2002). Plant Lipoxygenases. Physiological and Molecular Features. Plant Physol., 130 (1): 15-21.