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Mempro™ Detergent-Free Lipoxygenase Production

Based on the comprehensive protein engineering platform established through years of experience, scientists from Creative Biostructure offers tailored Mempro™ lipoxygenases production services using detergent-free expression system.

Lipoxygenases are a family of enzymes, most of the time they catalyze polyunsaturated fatty acids to the process of deoxidization reaction in lipids having a cis. The lipoxygenases is concerned with each other, as a result of their genetic construction and semblable dioxygenation activity.
Otherwise lipoxygenases are discover in animals, fungi and plants. Products of lipoxygenases are referring to miscellaneous cell functions. Lipoxygenases might possibly related to a great quantity of diverse sides of plant physiology, such as growth and development, senescence to wounding and the resistance of pest. They are most general in plants, however lipoxygenases isozymes are refer to the metabolism of eicosanoids in mammals. Nevertheless, ALOXE3, belongs to the lipoxygenases, has a lipoxygenase genetic construction but possesses rarely dioxygenation activity. The principal activity of ALOXE3 might be as an enzyme which catalyzes the transition of hydroperoxy unsaturated fatty acids to their 1,5-epoxide, hydroxyl derivatives.

Mempro™ Detergent-Free Lipoxygenase Production
Figure 1. The structural of rabbit reticulocyte 15s lipoxygenase (Proteins 2008).

Creative Biostructure has rich professional experience in high quality lipoxygenases production using detergent-free membrane protein expression system, we can perform various strategies for Mempro™ detergent-free protein production, including:

  • Mempro™ peptidase MA production using nanodiscs

A nanodisc can magically make membrane proteins stabilized, due to its structural characteristics, the membrane proteins in aqueous solutions were also solubled by nanodisc. In contrast with bicelles, detergent micelles and liposomes, a more native environment where proteins remain monodisperse and active can be offered.

  • Mempro™ peptidase MA production using amphipols

Amphipols are soluble proteins that can keep membrane proteins soluble in water without common surfactants. Amphipols were synthesized by forming amide bonds between octylamine and, sometimes, isopropylamine.

  • Mempro™ peptidase MA production using poly (styrene-co-maleic acid) lipid particles (SMALPs)

SMALPs are disc like structures containing a protein in a lipid bilayer, by using this approach, there is no need to sift special detergents, the speed and economy of protein extraction can be significantly improved. In addition, the use of SMALPs can make detergent-free isolation of membrane proteins and retention of their crude lipid environment available.

These novel detergent-free technologies for lipoxygenases production can be obtained easily, and enabling more comprehensively structural and functional studies.

Creative Biostructure provides other various Mempro™ membrane protein production services. Please feel free to contact us for a detailed quote.

References:
L. Kathy et al. (2015). Dissecting the Structure of Membrane Proteins. Genetic Engineering & Biotechnology News 35 (17): 16. 
J.Bowie et al. (2001). Stabilizing membrane proteins. Current Opinion in Structural Biology 11 (4): 397–402. 
M. Gaczynska, et al. (1994). Peptidase activities of proteasomes are differentially regulated by the major histocompatibility complex-encoded genes for LMP2 and LMP7. Proc. Nat. Acad. Sci. U. S. A., 91(20): 9213-9217.
M. Jamshad, et al. (2011). Surfactant-free purification of membrane proteins with intact native membrane environment. Biochem. Soc. Trans., 39: 813-818.
Lipoxygenases.


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