Creative Biostructure

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Protein Engineering and Analysis

Over the years, scientists from Creative Biostructure have been actively working on the characterization of proteins using a variety of techniques, as well as the engineering of proteins for various applications. The extensive experiences gained in these areas enable us to provide customized services in a timely and cost-effective manner.

Protein Analysis
With the rapid growth of genomic sequencing data, an increasing number of proteins have emerged with great research interest. To further understand their biological roles and pave the way for structure-function relationship studies, many efforts have been made toward the development of various methods that have been proved effective in the biophysical characterization of biomolecules. Creative Biostructure provides a variety of sensitive MagHelix™ techniques and the most appropriate strategies for protein analysis. The methods include but are not limited to:

Small angle X-ray scattering analysis of the peptide treatment Figure1. Small angle X-ray scattering analysis of the peptide treatment

With a full understanding of various techniques and the requirements of every client, scientists from Creative Biostructure will select appropriate strategies to assist your protein analysis project.

Protein Engineering
Protein engineering is the process of modifying the structures of proteins and assigns them new and/or desirable properties in terms of activity, solubility, affinity, stability, specificity, resistance, etc. The most commonly applied methods for protein engineering include de novo design, rational design and directed evolution. Creative Biostructure now provides unparalleled protein evolution and engineering services covering the full cycle of protein engineering to satisfy customers’ needs in the most cost-saving and time-effective manner.

Strategies for  protein engineering Figure 2. Strategies for protein engineering

Protein evolution can be described as the changes over time in protein shape, composition and structure. This process is closely related to the changes and selection of DNA polymorphisms and mutations due to protein sequence change responding to alteration in DNA sequence. Creative Biostructure adopts sensitive sequence profile methods and structure comparison methods to determine evolutionary relationships not detectable at the DNA-level. As for mutant library construction, we have strong expertise in de novo gene synthesis, which allows us to synthesize complex protein libraries without any dramatic increase in cost. With rational protein design, we make site-directed changes on the protein of interest based on structure-function relationship. Directed evolution is a method used to simulate the process of natural selection to evolve proteins or nuleic acids towards desired goals. Notably, Three factors are required for evolution: 1). The variation happens between replicators, 2). The variation causes fitness differences upon which selection acts, 3). The variation can be heritable.

In conclusion, Creative Biostructure provides specialized mutant library services, including site-directed mutagenesis libraries, sequential permutation scanning libraries, and randomized and degenerated libraries.

Please feel free to contact us for a detailed quote.

Ordering Process

Ordering Process

References

  1. Ryan TM, et al. (2013) Ammonium hydroxide treatment of Aβ produces an aggregate free solution suitable for biophysical and cell culture characterization. PeerJ. doi: 10.7717/peerj.73.
  2. Stepankova V. (2013) Strategies for Stabilization of Enzymes in Organic Solvents. ACS Catalysis 3(12):2823-2836.
  3. Kaumaya P. (2012) Protein Engineering: protein engineering methods and applications. ISBN 978-953-51-0037-9.
  4. Protein engineering. (https://en.wikipedia.org/wiki/Protein_engineering).

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