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Optimization of Screening Kits and Reagents

Protein Crystallization Tools Creative Biostructure’s optimization screening is designed to assist in the identification of solution conditions, which promote protein solubility, stability and minimize protein precipitation.

Optimization screening is to screen for the solubility, stability and the consequent crystallization, even in the presence of a crystallization reagent. Altogether, there are 94 different chemical additives sampling 17 different classes plus two controls.

96 sterile filtered reagents
17 classes of reagents and excipients
Highly concentrated (10x) reagent formulation
Highly compatible with other protein assays
Thermal shift assay
Dynamic Light Scattering
Use Solubility & Stability Screen alongside Slice pH and Solubility & Stability Screen to optimize the sample buffer

Protein solubility and stability are required in an extensive range of applications, including genetic-proteomics research, the preparation of proteins in pharmaceuticals, structural biology and crystallization analysis.

  • Sodium hydroxide [NaOH; Mr 40.00]
Optimization Polymers
Ethylene imine polymer solution (50% solution - 200 ml)
Polyethylene glycol (200/400/600/1500/4000/6000/8000)
Pentaerythritol ethoxylate (3/4 EO/OH; 15/4 EO/OH; 5/4 PO/OH; 17/8 PO/OH)
Polyethylene glycol monomethyl (550/2000/5000)
Optimization Salts
Ammonium acetate [C2H7NO2CH3COONH4; Mr 77.08]
Ammonium chloride [NH4Cl; Mr 53.49]
Ammonium citrate dibasic [(NH4)2C6H6O7; Mr 226.19]
Ammonium fluoride [NH4F; Mr 37.04] 
Ammonium phosphate dibasic [(NH4)2HPO4; Mr 132.06]
Cadmium sulfate hydrate [CdCl2-xH2O; Mr 183.32] 
Calcium chloride dehydrate [CaCl2·2H2O; Mr 147.01]
Cesium chloride [CsCl; Mr 168.36]
Potassium sodium tartrate tetrahydrate [C4H4KNaO6·4H2O; Mr 282.22]
Tryptone [Microbiologically tested]
Sodium citrate tribasic dehydrate [C6H5Na3O7·2H2O; Mr 294.10]
Trimethylamine N-oxide dehydrate [(CH3)3NO·2H2O; Mr 111.14]
Zinc acetate dehydrate[Zn(CH3COO)2·2H2O; Mr 219.52 ]
Zinc sulfate heptahydrate [ZnSO4·7H2O; Mr 287.56]
Optimization Buffers
Acetic acid [CH3CO2H; Mw 60.05]
ADA Buffer [N-(2-Acetamido)iminodiacetic acid; N-(Carbamoylmethyl)iminodiacetic acid]
BICINE Buffer [N, N-Bis(2-hydroxyethyl)glycine]
HEPES sodium Buffer [4-(2-hydroxyethyl) piperazine-1-ethanesulfonic acid sodium salt]
HEPES  [4-(2-Hydroxyethyl) piperazine-1-ethanesulfonic acid]
Imidazole Buffer [1,3-Diaza-2, 4-cyclopentadiene and Glyoxaline]
Tris Buffer [Trizma base or THAM or Tris base]
TRIS hydrochloride Buffer [TRIS HCl, Trizma hydrochloride]
Sodium citrate tribasic dihydrate Buffer [HOC(COONa)(CH2COONa)2·H2O ]

Creative Biostructure’s optimization reagents can make your crystallization screening test more accurate and reproducible.