Mempro™ Detergent-Free Galactose-Binding Domain-Like Protein Production

Creative Biostructure can provide custom Mempro™ galactose-binding domain-like protein production services using detergent-free expression system.

Galactose-binding domain-like proteins are a family of proteins that have carbohydrate binding activity, which can be found in both eukaryotes and prokaryotes. Notably, the featured structure of galactose-binding domain is a beta-sandwich. Members from galactose-binding domain-like family share a high similarity in the beta-sandwich and in the loops. The biological funtion of galactose-binding domain-like proteins is to bind bind carbohydrate and have carbon-oxygen lyase activity. And the galactose-binding domain-like proteins are involved in carbohydrate metabolic process. Conventional procedures for membrane protein production require extraction from the membrane into detergent micelles. However, the drawback of detergent is that can result in conformational changes on the proteins. Our alternative detergent-free expression system can best preserve the native conformation of galactose-binding domain-like proteins for further functional analysis.

Figure 1. Lactadherin, a member of galactose-binding domain-like family. (OPM database)

Creative Biostructure has extensive expertise in high-yield galactose-binding domain-like protein production in detergent-free protein expression system, we can perform various strategies for Mempro™ detergent-free galactose-binding domain-like protein production, including:

• Mempro™ galactose-binding domain-like protein production using nanodiscs

  Nanodiscs are the self-assembled technique to stabilize transmembrane proteins removed from the membrane by membrane scaffold proteins (MSPs) without detergent. Creative Biostructure can reconstitute galactose-binding domain-like proteins into nanodiscs in detergent-free procedure: Expressing the galactose-binding domain-like proteins in the cell-free expression system with the addition of pre-assembled nanodiscs.

• Mempro™ galactose-binding domain-like protein production using amphipols

  The amphipathic amphipols have the ability to enwrap transmembrane proteins, allowing them to stay folded. Creative Biostructure can apply A8-35 to solubilize galactose-binding domain-like proteins during purification and stabilization processes.

• Mempro™ galactose-binding domain-like protein production using poly (styrene-co-maleic acid) lipid particles (SMALPs)

The SMALPs can be self-assembled by the simple addition of the SMA co-polymers. At neutral or alkaline pH, The disc-like structure assembles itself, encapsulating galactose-binding domain-like proteins in a formation amenable to be purified.

In sum, these novel detergent-free techniques for membrane protein production can be obtained easily, and enabling more comprehensively structural and functional studies.

Creative Biostructure provides other various Mempro™ membrane protein production services. Please feel free to contact us for a detailed quote.

References:
C. Tribet, et al. (1996). Amphipols: Polymers that keep membrane proteins soluble in aqueous solutions. Proc Natl Acad Sci U. S. A. 93: 15047-15050.
Galactose-binding domain-like (IPR008979). (http://www.ebi.ac.uk/interpro/entry/IPR008979)
Galactose-binding domain-like protein. (http://www.uniprot.org/uniprot/K2RZ34)
M. Jamshad, et al. (2011). Surfactant-free purification of membrane proteins with intact native membrane environment. Biochem. Soc. Trans., 39: 813-818.