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Protein Crystallization and Structure Determination

Our understanding of life at the molecular level is highly dependent on the ability to map the molecular details of individual proteins and nucleic acids as well as their interactions with each other and with small molecules (inhibitors, cofactors, substrates, etc.). The determination of 3-D structures of proteins is crucial for the understanding of these interactions as well as their structure–function relationships, which also has many practical applications in drug design and protein engineering.

Structure determination is usually a tedious and expensive process whereby the target macromolecule must be produced in relatively large quantities and purified in high concentrations. A successful experiment often requires expert knowledge of biochemistry, bioinformatics and molecular biology. Over the past decades, rapid technical advances in DNA cloning, protein expression and purification methodologies have made it generally easy to obtain enough amount of proteins for their structural study. Besides, numerous innovations and improvements have been made in solving protein structures using instrumental methods such as X-ray crystallography, nuclear magnetic resonance (NMR) spectroscopy and electron microscopy (EM). These efforts together have given rise to dramatic accumulation of 3-D structural data in the past few decades (Figure 1). The large number of protein structures will certainly yield valuable information to the rules for elucidating protein folding/unfolding mechanisms and understanding their biological functions.

he growth  of total available structures deposited in PDB since 2000. Figure 1. The growth of total available structures deposited in PDB since 2000.

Creative Biostructure has a team of scientists who have rich experience in solving the structure of various protein targets, such as kinases, polymerases, chaperones and membrane proteins. Over the years, we have developed four unparalleled platforms, including X-ray crystallography, NMR, EM and membrane protein screening, to facilitate your research in structural biology.

Figure 2. Four platforms at Creative Biostructure

By using the most advanced platform apparatus and techniques, Creative Biostructure promises unbeatable precision in protein structure services with competitive prices.

  • Protein crystallization: Creative Biostructure offers high-throughput UniCrys™ protein crystallization services with our state-of-the-art facilities. High-quality protein crystals can be obtained, which are suitable for X-ray diffraction studies.
  • Structure determination: Creative Biostructure employs various strategies for the structure determination of macromolecules of your interest. Our scientists are experienced with phase determination of X-ray diffraction pattern, NMR spectroscopy and cryo-EM. We promise to provides the most efficient and economical strategies tailored to our customers’ requirement.

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