Isothermal Titration Calorimetry (ITC) for Coronavirus Research

Isothermal titration calorimetry (ITC) is widely used to detect the affinity and enthalpy of interactions between proteins and/or other molecules. In addition, the quantitative nature of ITC is useful in the characterization of recombinant proteins, as well as to determine the ratio of proteins capable of binding specific ligands, thus determining protein purity. Creative Biostructure rapidly integrates expertise and related businesses, providing high-quality ITC services for applications such as biomolecular interactions involved in coronavirus infection research.

Brief Introduction to Isothermal Titration Calorimetry (ITC)

Both intramolecular and intermolecular biochemical reactions involve a reversible heat-driven process, that is, a certain degree of heat change occurs before and after the reaction. The ITC technology utilizes the principle of power compensation, which can accurately obtain the intramolecular/intermolecular interactions of the system in a wide concentration range by single-concentration scanning, directly measure the heat of the reaction, and complete the fitting calculation of combined thermodynamic parameters. By fitting the interaction mode of the two reactants with the software, it is possible to directly calculate the reaction binding enthalpy (ΔH), the number of binding sites, binding equilibrium constant (Ka), constant pressure heat capacity (ΔCp), as well as kinetic data between two or more molecules in the solution. Thus, we can derive the Gibbs free energy (ΔG) and entropy change (ΔS) of the reaction.

The basic principle of ITC technology.  Figure 1. The basic principle of ITC technology. (Song C.; et al. 2015)

Our ITC Services for Coronavirus Research

The research team of Creative Biostructure with a shared scientific background in the fields of virology and biophysics and provides custom ITC assay development and detection services for coronavirus infection, including COVID-19. We support in experimental design, assay development, verification, validation, and data analysis. ITC technology has the advantages of label-free, wide dynamic range, rich information, and easy to use, which makes it possible to perform a variety of experiments with ITC and obtain various experimental purposes. At Creative Biostructure, we can use ITC technology to help you achieve the research objectives of coronavirus infection, such as:

  • Determine enzyme kinetics
  • Measure target activity
  • Confirm the combination of drug and target
  • Using thermodynamics to guide the optimization of lead compounds
  • Explore the mechanism of action
  • Confirm IC50 and EC50 values
  • Quickly get KD values in secondary screening or hit verification

Creative Biostructure has established diverse approaches based on advanced technologies for analysis of biomacromolecules interactions. Want to know more? Please feel free to contact us for more information. Our customer service representatives are available 24 hours a day from Monday to Sunday.

Contact us to discuss your project!

Reference

  1. Song C.; et al. Choosing a suitable method for the identification of replication origins in microbial genomes. Frontiers in microbiology. 2015, 6: 1049.

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