Determining the three-dimensional (3D) structure of proteins is critical for understanding the mechanism of coronavirus infection, discovering potential drug targets and structure-based drug design. Rapid advances in methodologies for protein expression and purification have made it easy to obtain enough quantities of proteins for structural studies. In addition, many breakthroughs have been made in X-ray crystallography, nuclear magnetic resonance (NMR) spectroscopy, and electron microscopy (EM) techniques related to protein structure analysis. As a professional structural biology service supplier, Creative Biostructure can offer a range of services for the structure determination of viral structural proteins/non-structural proteins, host proteins, antigen-antibody complexes, etc., using our three established structural biology technology platforms.
Coronavirus (CoV) is an enveloped virus with a positive-strand RNA genome. It belongs to the Nidovirales order of the Coronaviridae family and is divided into four genera (α, β, γ, and δ). And the new coronavirus involved in the recent outbreak belongs to the β genera. To date, no specific therapeutic drug or vaccine has been approved for the treatment of human coronavirus. Coronavirus infection is a sophisticated process that requires the synergistic effects of receptor binding and proteolysis of the spike (S) protein to promote virus-cell fusion. 3D structure information of proteins can show the details of the interface between virus and host and contribute to guiding the design of vaccines and the discovery of drugs against human coronavirus.
Figure 1. Structural studies of human Coronavirus strains binding to host receptors. (Adapted from Tortorici M A.; et al. 2019)
Creative Biostructure has comprehensive structural biology research capabilities. For coronavirus research, we can provide a series of structural biology services for protein structure determination, from molecular cloning to data interpretation. Our services include but not limited to:
Creative Biostructure utilizes a variety of strategies to determine the structure of the macromolecules you are interested in coronavirus research, to promote research on the pathogenic mechanisms of coronaviruses and the development of antiviral therapies. We guarantee: