Proteins are the executors of coronavirus infection in host cells. To understand the functions of specific proteins during infection, we need to study many aspects of proteins, such as protein interactions, protein modifications, and three-dimensional (3D) structures of proteins. In general, the premise of protein structural analysis is to obtain the target protein with high purity. For different applications and proteins with different properties, different protein expression and purification techniques are required to be adopted to obtain high-quality proteins. Nowadays, Creative Biostructure provides custom services for the expression and purification of soluble/membrane proteins involved in coronavirus research.
The name of coronavirus is derived from the crown-like morphology observed under the electron microscope. Before the discovery of novel coronavirus (SARS-CoV-2, formerly called as 2019-nCoV), there were six known human coronaviruses. Four of these coronaviruses are more common in humans and are less pathogenic, generally causing only mild respiratory symptoms similar to the common cold. Two other coronaviruses, SARS-CoV and MERS-CoV, can cause Severe Acute Respiratory Syndrome and Middle East Respiratory Syndrome, respectively. The novel coronavirus that began to outbreak in December 2019 is a new virus strain that has never been found in humans before, causing a severe respiratory disease termed as COVID-19.
Our services are tailored to the specific needs of academic research or industrial applications, emphasizing the development of the appropriate "gene-to-protein" process for different projects. Our technology platform supports every process of your target protein expression and purification project, including but not limited to, gene synthesis, protein expression, protein modification, mutagenesis, protein purification and characterization.
Over the past decade, Creative Biostructure has established comprehensive expression systems to produce recombinant proteins, including cell-based expression systems (bacterial, yeast, insect, and mammalian cells) as well as cell-free expression systems. For membrane protein isolation, we have developed other cutting-edge systems, including technologies of liposome, Nanodisc, and virus-like particle.
At Creative Biostructure, we are confident that we can provide purification strategies to obtain high-purity target proteins and continuously optimize purification schemes to improve yield and purity. Our purification platform includes a variety of advanced chromatography and other techniques, such as affinity chromatography, ion-exchange chromatography, size-exclusion chromatography, hydrophobic interaction chromatography, reversed phase chromatography, etc.
Each expression system and purification method have advantages and disadvantages. The scientists of Creative Biostructure will optimize the protocol according to the properties and application of the target protein, and finally choose the optimal solution to obtain high-quality protein, so as to meet your needs for determining the high-resolution 3D structure of proteins involved in coronavirus research and accelerating antiviral therapeutics.