Background

Homo sapiens L-alpha-glycerol-3-phosphate dehydro-genase 1 (GPD1) catalyzes the reversible biological conversion of dihydroxyacetone (DHAP) to glycerol-3-phosphate. The GPD1 protein was expressed in Escherichia coli, and purified as a fusion protein with glutathione S-transferase.

Protein Classification

Oxidoreductase

Structure Weight

39164.02 Da

Polymer

1

Molecule

Glycerol-3-phosphate dehydrogenase [NAD+], cytoplasmic

Chain Length

354 amino acids

PDB ID

1X0X

MMDB ID

38469

Source

E.coli

Method

X-Ray Diffraction

Resolution

2.75Å

Ligand Chemical Component

NAD; Sulfate ion

Gene Name

GPD1

Synonyms

FLJ26652; glycerol-3-phosphate dehydro-genase, soluble; glycerol-3-phosphate dehydrogenase 1 (soluble); EC 1.1.1.8; GPD-C; GPDH-C

UniProt ID

P21695

Gene ID

2819

Chromosome Location

12q12-q13

Function

NAD or NADH binding; binding; glycerol-3-phosphate dehydrogense (NAD+) activity; glycerol-3-phosphate dehydrogense activity; protein homodimerization activity

Reference

Ou, X., Ji, C., Han, X., Zhao, X., Li, X., Mao, Y., Wong, L.L., Bartlam, M., Rao, Z. (2006) Crystal Structures of Human Glycerol 3-phosphate Dehydrogenase 1 (GPD1) J.Mol.Biol. 357: 858-869