X-ray crystallography is currently the most favored method for structural determination of proteins and other macromolecules. The requisite for a successful X-ray crystallographic application is to obtain single crystals of the target protein. Data is then collected by diffracting X-ray from the single crystal that has an ordered pattern of atomic orientation. The assembly of atoms and molecules in the crystal can be deduced from the measurement of X-ray scattering.
At present, more than 120,000 protein structures resolved by X-Ray crystallography experiments have been deposited in protein databank, accounting for nearly 90% of the resolved proteins, suggesting a predominant popularity of X-Ray crystallography in structural determination.
Fig.1. The growth of structures from X-ray crystallography experiments deposited in PDB
Creative Biostructure provides protein crystallization and X-Ray crystallography services in our state-of-the-art facilities, and has developed an X-ray crystallography pipeline that covers all technical stages from gene synthesis to structure determination. Our experienced scientists work closely with the clients to ensure rapid turnaround and reliable results.
Key features of this service?
What We Do — Integrated Gene to Structure Services
Step 1: Construct design and expression optimization
Step 2: Crystallization-grade protein purification
Step 3: Protein crystallization and optimization
Step 4: X-ray screening and dataset collection
Step 5: Data analysis and Structure determination
Creative Biostructure not only conducts quality X-Ray crystallography services for customers all over the world, but also provides de novo preparation of protein crystals upon request. Our service plans come with well-defined methodologies and instruments, yet offer a great deal of flexibility. Please contact us for more information.
Creative Biostructure can provide a variety of crystallization strategies, particularly for membrane protein crystallization. The detailed services are summarized as follows.
Providing a more bilayer-like environment for membrane proteins than in detergent micelles, enabling the use of standard crystallization screening methodology for membrane proteins.
Improvement of protein solubility and crystallization assisted by mutant library construction and screening.
Crystal optimization solutions
Your specific sample types for crystallization
Please feel free to contact us to discuss your project!