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Advanced Protein Production and Engineering Services
Struggling with challenging protein targets? Our comprehensive platform excels in membrane proteins and custom engineering, providing high-purity proteins, enhanced properties, and detailed characterization data to drive your research success with proven expertise.
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The ability to produce and engineer proteins allows researchers to explore protein function in a controlled setting, as well as create novel proteins with optimized properties for drug discovery, industrial catalysis, and biotechnological applications. High-quality proteins are fundamental for studying protein-protein interactions, mapping epitopes, and determining 3D structures.
However, researchers encounter challenges including insufficient expression levels, protein misfolding, protein aggregation, and limited stability under experimental conditions. Moreover, the growing complexity of protein engineering projects, such as designing novel binding interfaces and optimizing catalytic properties, demands sophisticated technical approaches beyond conventional methods. Our comprehensive protein services platform addresses these challenges through advanced expression systems, innovative purification strategies, and rational protein engineering approaches.
Recombinant Protein Production
Our recombinant protein production services offer comprehensive and customizable solutions from gene to purified protein. We provide expertise across multiple expression systems such as E. coli, mammalian cells (CHO, HEK293), and insect cells (Sf9/Hi5), enabling optimal production strategies for diverse protein types. Our integrated workflow encompasses codon optimization, expression condition screening, and multi-step purification development. We deliver both research-grade proteins for basic research and structural studies, as well as GMP-grade materials for downstream applications. Each production process can be tailored to meet specific requirements for yield, purity, activity, and scale.
Specific Solutions
- Bacterial Protein Expression System (E. coli, Lactococcus lactis, Bacillus subtilis, Rhodobacter sphaeroides, etc.)
- Mammalian Protein Expression System (CHO, HEK293, BHK-21, COS-7, etc.)
- Insect Expression System (Sf9, Sf21 and Hi5)
- Yeast Expression System (P. pastoris and S. cerevisiae)
- Plant Cell Expression System
- Cell-free Protein Expression System
Technical Details (Partial)
- Strain screening and optimization
- Vector design and construction
- Expression validation
- System-specific protocol development
Specific Solutions
- Gene Design & Synthesis
- Codon Optimization
- Fusion Tag Strategy
- Expression Condition Screening
Technical Details (Partial)
- Sequence analysis and optimization
- Tag selection (His, GST, MBP, etc.)
- Temperature/induction optimization
- Media composition optimization
- Expression level analysis
Specific Solutions
Technical Details (Partial)
- Protocol development
- Process optimization
- Scale-up strategy
- Buffer optimization
- Method validation
Specific Solutions
- Purity Analysis
- Activity Testing
- Endotoxin Detection
- Stability Assessment
- Structural Analysis
Technical Details (Partial)
- SDS-PAGE/Western Blot
- SEC-MALS
- Thermal stability testing
- Mass spectrometry
- Functional assays
- Structural biology techniques
Specific Solutions
- Research Grade Production
- GMP Protein Process Development and Manufacturing
- Different Scale Options
Technical Details (Partial)
- mg to gram scale production
- Quality control documentation
- Batch consistency validation
- Storage and stability studies
Our Core Expertise — Membrane Protein Production
With specialized expertise in membrane protein production, we excel at expressing and purifying complex targets, including GPCRs, ion channels, and transporters, using optimized detergent screening and stabilization strategies. Our advanced technical platforms and extensive experience enable us to address various membrane protein challenges through a comprehensive suite of services—from cell-based and cell-free expression systems to both traditional detergent-based and innovative detergent-free methods. Our customizable solutions are tailored to ensure protein functionality and stability, meeting diverse research needs.
| Service Category | Specific Solutions | Features | Applications |
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| Protein Expression | Cell-based Membrane Protein Expression |
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| Cell-free Membrane Protein Expression |
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| Animal-free Membrane Protein Expression |
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| Protein Purification | Detergent-based Membrane Protein Purification |
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| Detergent-free Membrane Protein Purification |
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| Plasma Membrane Protein Isolation |
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| Protein Stabilization | Nanodiscs Construction |
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| Amphipol Reconstitution |
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| Liposome Reconstitution |
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Contact us to discuss your project!
Advanced Membrane Protein Products
Creative Biostructure offers comprehensive custom membrane protein production services, encompassing transmembrane protein production, monotypic/peripheral protein production, and membrane protein peptide production.
Leveraging the latest technologies, we also provide expertly assembled nanodiscs with embedded membrane proteins, ensuring enhanced protein stability and functionality in a native-like environment.
Nanodisc Human CD20/MS4A1
Protein
Nanodisc Human CD133 Protein
Nanodisc Human
Claudin-18.2 Protein
Nanodisc Human GPRC5D
Protein
Nanodisc Human
Claudin-6/CLDN6 Protein
View More Assembled Nanodiscs →
Additionally, we have developed a portfolio of ready-to-use membrane proteins in lipid vesicles via a cell-free expression system, offering clients reliable access to high-quality, advanced membrane protein products for diverse research applications.
View More Membrane Protein Liposomes →Mastering Protein Engineering from Evolution to Design
Our protein engineering services seamlessly integrate with our protein production expertise, offering comprehensive solutions for protein optimization and modification. We combine rational design, directed evolution, and computational approaches to engineer proteins with enhanced properties – from improved stability and solubility to optimized activity and binding affinity. Our services span from targeted mutagenesis to complex protein evolution studies, enabling the development of proteins with tailored characteristics for research, industrial, and biotechnology applications. Whether complementing our protein production services or as standalone solutions, our engineering capabilities are backed by advanced computational tools, high-throughput screening platforms, and extensive expertise in protein structure-function relationships.
| Service Category | Specific Solutions | Methods & Technologies (Partial) |
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| Protein Evolution Services |
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| Custom Mutagenesis Services |
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| Epitope Mapping Services |
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| Advanced Characterization |
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| Computational Analysis |
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Native Protein Isolation and Purification
Our native protein purification services leverage advanced isolation and purification technologies to extract proteins from diverse biological sources while maintaining their natural structure and function. We offer comprehensive solutions for purifying proteins from various sources including microbial cultures, cell lines, plant tissues, and biological materials. Our expertise spans from initial sample processing to final purification, ensuring high purity and biological activity for applications in research, industrial processes, and biotechnology innovations. With customizable protocols and rigorous quality control, we deliver native proteins suitable for various scientific and industrial applications.
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Ready to Advance Your Protein Research?
Talk With An Expert →Contact our experts to discuss your protein challenges and receive a customized quote - whether you need standalone services or integrated solutions, we'll design the optimal strategy to drive your research forward.
Instrument Platform
Creative Biostructure is equipped with advanced instruments throughout the entire protein production and characterization workflow. For protein expression, we operate advanced bioreactor systems, automated cell culture platforms, continuous perfusion systems, and microfluidic cell culture devices. For protein purification and separation, we utilize ÄKTA protein purification systems with various chromatography options. Our analytical capabilities include high-resolution mass spectrometers (LC-MS/MS), multi-angle light scattering (MALS) detector, surface plasmon resonance (SPR) analyzer, bio-layer interferometry (BLI) system, and circular dichroism (CD) spectrometer. For protein characterization, we employ differential scanning calorimetry (DSC), dynamic light scattering (DLS) analyzer, and automated high-throughput screening platforms. Our structural biology studies are supported by cryo-electron microscopy (Cryo-EM), X-ray crystallography, and NMR spectroscopy facilities. Additionally, we maintain automated protein crystallization systems and robotic liquid handling workstations for high-throughput applications.
Featured Case Studies of Protein Production Services
* NOTE: We prioritize confidentiality in our services to safeguard technology and intellectual property
for enhanced future value and protection. The following case study has been shared with the client's
consent.
Custom Protein Production and Nanodisc Assembly for F2 Receptor
The project aimed to produce and stabilize the F2 receptor membrane protein by assembling it within a Nanodisc. This method sought to improve the protein's solubility and structural stability, ensuring it remained functional and available for downstream applications.
In this case study, our innovative approach to optimizing membrane protein production and nanodisc assembly provided a robust solution for stabilizing the F2 receptor. By fine-tuning each step from expression to purification, we delivered a high-purity, stable protein-nanodisc complex tailored to the client's specific needs. This case demonstrates our ability to adapt protein production processes to meet the specific requirements of complex membrane proteins, providing clients with reliable, high-purity protein solutions for advanced applications.
Figure 1. Detection of F2 receptor in supernatant across Nanodiscs concentration gradient. Lanes 1-7: F2-nanodiscs complex with Nanodisc concentrations of 10, 20, 40, 60, 80, 100, and 120 µM, respectively. Lane 8: supernatant negative control. Lane 9: F2 control (reaction system with only F2 expression plasmid). Red arrows indicate the target membrane protein F2 (42.9 kDa), and the yellow arrow indicates the Nanodiscs scaffold protein (26 kDa).
Figure 2. Detection of F2 receptor in precipitation across Nanodiscs concentration gradient. Lane 1: F2 control (reaction system with only F2 expression plasmid). Lanes 2-8: F2-nanodiscs complex with Nanodisc concentrations of 10, 20, 40, 60, 80, 100, and 120 µM, respectively. Red arrow indicates the target membrane protein F2 (42.9 kDa), and yellow arrow indicates the Nanodiscs scaffold protein (26 kDa).
Figure 3. SDS-PAGE analysis of purified F2-nanodiscs complex. The red arrow indicates the target membrane protein F2 (42.9 kDa), and the yellow arrow indicates the Nanodiscs scaffold protein (26 kDa).
Figure 4. Western blot analysis of purified F2-nanodiscs complex. The red arrow indicates the target membrane protein F2 (42.9 kDa).
Case Study
Custom Protein Production and Nanodisc Assembly for F2 Receptor
This poster presents the complete case study content, including background, methods and materials, results, conclusions and discussion.
- cDNA Optimization and Cloning
- Nanodisc Assembly and Optimization
- Purification and Quality Verification
Download PDF
Isolation and Purification of Native R1 Complex
This project aimed to isolate and purify the R1 protein complex from tissue samples. The R1 complex, composed of four subunits (A, B, C, and D), required an optimized method to ensure high purity and stability for downstream applications.
We provided expertise in antibody-conjugated affinity chromatography, enabling the successful isolation of the R1 protein complex with high specificity and purity. By customizing the conjugation and purification steps, we delivered a purified, structurally stable R1 complex.
Figure 1. Western blot analysis of mouse B protein in tissue samples. The red arrow indicates the target protein band. Lane M: Marker; Lane 1: Kidney PBS sample; Lane 2: Kidney RIPA sample; Lane 3: Kidney cell lysate; Lane 4: Liver PBS sample; Lane 5: Liver RIPA sample; Lane 6: Liver cell lysate; Lane 7: Skin PBS sample; Lane 8: Skin RIPA sample; Lane 9: Skin cell lysate; Lane 10: Spleen PBS sample; Lane 11: Spleen RIPA sample; Lane 12: Spleen cell lysate.
Figure 2. SDS-PAGE analysis of anti-B antibody conjugation on beads. Lane 1: Antibody solution before conjugation; Lane 2: Antibody solution after conjugation; Lane M: Marker.
Figure 3. SDS-PAGE and Western blot analysis of purified R1 complex. Left: SDS-PAGE; Right: Western blot. The red arrow indicates the target protein. Lane M: Protein Marker; Lane FT: Flow-through; Lanes W1-W2: Wash; Lanes E1-E3: Elution.
Case Study
Isolation and Purification of Native R1 Complex
This poster presents the complete case study content, including background, methods and materials, results, conclusions and discussion.
- Antibody Conjugation to Beads
- Protein Complex Purification
- Western Blot Analysis
Download PDF
Why Choose Creative Biostructure?
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Excellence in Protein Production
: With extensive expertise in both conventional and challenging proteins, we offer optimized solutions for prokaryotic, mammalian, and cell-free expression systems. Our strength in membrane protein production, supported by cutting-edge technologies, ensures exceptional protein quality and yield. -
Advanced Protein Engineering Platform
: Our comprehensive protein engineering platform integrates directed evolution, rational design, and high-throughput screening capabilities. From custom mutagenesis libraries to sophisticated protein evolution strategies, we provide data-driven solutions for optimizing protein properties and functions. -
Specialized in Challenging Targets
: We excel in handling challenging proteins, particularly membrane proteins and complex protein assemblies, through innovative approaches including advanced detergent screening, protein stabilization technologies, and detergent-free systems. -
Customized Project Design
: Every project receives a tailored strategy based on specific requirements. Our flexible service model allows clients to choose from standalone services to integrated solutions, with adjustable scales from milligrams to grams.
"Our collaboration involved a complex membrane protein project. Their expertise in handling membrane proteins shone through, from tailor-made detergent selection to protein characterization. Their regular updates and adaptability to refine protocols based on preliminary outcomes were highly beneficial. The high-quality final protein product surpassed our expectations, facilitating our structural analysis. Their technical proficiency and problem-solving skills greatly hastened our research progress."
— Dr. Michael C., Senior Research Scientist, Institute of Molecular Biology
"Creative Biostructure enhanced our industrial enzyme's stability and activity through a blend of rational design and directed evolution. The high-throughput screening platform they custom-built for our project was highly effective. Their meticulous documentation and comprehensive data analysis clarified the optimization process. The resulting enzyme exhibited a fourfold stability boost and a 2.5-fold activity increase."
— Dr. Sarah A., Principal Investigator, Department of Biochemistry
"This team consistently delivers high-quality proteins, even with challenging targets. What sets them apart is their systematic approach and attention to detail. Using their automated platforms, they efficiently optimized expression conditions for multiple proteins simultaneously. Each batch comes with comprehensive analysis and documentation. Their technical expertise and consistent quality have made them our long-term partner for protein production."
— Prof. Allen S., Head of Protein Science
Protein Production and Engineering FAQs
What is the typical timeline for membrane protein
production, from gene to purified protein?
For a typical membrane protein project, the complete process usually takes
6-8 weeks. This includes 2-3 weeks for expression system optimization and initial trials, followed by
3-4 weeks for purification optimization and final production. However, timeline can vary based on
protein complexity and specific requirements. For challenging targets requiring extensive optimization,
we'll provide a customized timeline during initial consultation.
How do you determine the most suitable expression system
for my protein?
Our selection process is based on comprehensive analysis of protein
characteristics, including size, modifications required, and complexity. We conduct parallel small-scale
expression trials in multiple systems (E. coli, mammalian cells, insect cells) to evaluate expression
level, protein quality, and functionality. This systematic approach allows us to quickly identify the
optimal expression system for your specific protein.
What strategies do you employ when protein engineering
projects face solubility or stability issues?
We implement an integrated approach combining computational analysis with
experimental optimization. This typically involves structure-guided modifications, systematic
mutagenesis, and high-throughput stability screening. Our advanced protein engineering platform enables
rapid identification of optimal conditions and mutations. We continuously adjust our strategy based on
real-time results to achieve the desired protein properties.
What quality control measures and documentation do you
provide with the final protein?
Each protein batch comes with a comprehensive analysis package including
purity assessment (SDS-PAGE, SEC), identity confirmation (Mass Spectrometry), homogeneity analysis
(DLS), and project-specific functional assays. We provide a detailed production report containing all
critical parameters and methods. Additional analyses can be included based on your specific
requirements.
Can you handle both small-scale protein production for
research and larger-scale production?
Our flexible production platform accommodates various scales, from
milligram quantities for initial research to gram-scale production when needed. We develop optimized
processes that maintain consistency across different scales, supported by our comprehensive monitoring
systems. Our experienced team ensures smooth transition between scales while maintaining protein quality
and functionality.
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