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Exosome Analysis and Characterization

Creative Biostructure’s exosome analysis and characterization service makes it easy to profile exosomal lipids, proteins and RNAs with our years of experience working on exosomes. Our expertise provides comprehensive support for your exosome engineering study by including the following customized services.

The Importance of Exosome Analysis and Characterization

Characterization of exosomes has become a topic of interest in medical research and molecular biology because it can provide very valuable information for early detection, disease monitoring and development of effective treatments against cancer and autoimmune diseases. Exosomes are known as one type of secreted membrane vesicles that are responsible for intercellular communication by providing means for intercellular exchange of proteins, lipids, mRNA, miRNA and DNA, contributing to intercellular communication in relevant biological processes, playing an important role in the development of several diseases, and specifically, modulating cancer microenvironment and the immune response. Exosomes and its contents also have emerged as non‐invasive biomarkers for various diseases. Exosomes composition and quantity may be directly related to the pathological state of the patient, so the characterization of exosome could be of great relevance for diagnosis.

Therefore, to acquire a deeper knowledge of the exosome biology and accelerate the use of these extracellular vesicles in diagnostic tests or clinical research, Creative Biostructure provides a sensitive, reproducible methods for exosome characterization.

Secretion of exosomes from cells and their significance for biomarkers. a) biogenesis of exosomes from multivesicular endo-somes, in contrast to the microvesicles which are released from the cell surface via membrane budding. b) exosomes and microvesicles both contain transmembrane proteins, intracellular proteins, DNA, RNA and miRNA, which are potential biomarkers. c) exosomes are found in all body fluids.Figure 1. Secretion of exosomes from cells and their significance for biomarkers. a) biogenesis of exosomes from multivesicular endo-somes, in contrast to the microvesicles which are released from the cell surface via membrane budding. b) exosomes and microvesicles both contain transmembrane proteins, intracellular proteins, DNA, RNA and miRNA, which are potential biomarkers. c) exosomes are found in all body fluids. (Sitar, Simona., et al. (2015) Anal. Chem. 87, pp. 9225-9233.)

Exosomal Lipidomics & Metabolomics Services

Lipids, an important part of cellular physiology, are the major components of exosomes.  The importance of lipids in exosome biology has been associated with the fact that lipid content of exosomes differs from the parent cells and also changes during physiological processes. Exosome lipidomics study provides a great access to identify lipid-based biomarkers and unravel its monitoring function in diseases. Besides, a wide range of metabolites in exosomes could also be used for biomarker discovery by showing distinct metabolite populations during different disease treatment stages.

Our customized services provide comprehensive support for exosomal lipidomics & metabolomics study by using different analysis methods for data acquisition, analysis, and even interpretation: Thin layer chromatography (TLC), High performance liquid chromatography (HPLC), NMR spectroscopy, Mass spectrometry, etc.

Exosomal Proteomics Services

Like exosomal lipidomics, exosomal proteomics also have been recognized an easy access for biomarkers monitoring, providing information on the physiology study of the parent cells and exosomes. For analysis by mass spectrometry, exosomal proteins can be separated by SDS-PAGE, in gel digested, followed by LC-MS/MS experiments. Based on our customer need, we can work from exosome isolation to data collection and analysis, a final report with results will be provided.

Exosomal RNA Sequencing Service

Nucleic acid is another important cargo that exosomes carry, including deoxynucleic acids (DNA), coding and non-coding ribonucleic acid (RNA) like messenger RNA (mRNA) and microRNA (miRNA). After exosome isolation, nucleic acids need to be accurately quantified in order to track many kinds of medical conditions and diseases, especially when nucleic acids of exosomes are greatly different from the parental cell content.

Creative Biostructure provides a complete exosome workflow solution to solve nucleic acids profile of exosomes, starting from fast and efficient isolation of RNA to exosomal RNA sequencing using qRT-PCR and novel sequencing techniques.

Based on the comprehensive techniques in exosome analysis and characterization, Creative Biostructure offers top services to satisfy your demands on exosome study. Please contact us for more information.

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References

  1. Sitar, Simona., et al. (2015) Size Characterization and Quantification of Exosomes by Asymmetrical-Flow Field-Flow Fractionation. Anal. Chem. 87, pp. 9225-9233 DOI: 10.1021/acs.analchem.5b01636.
  2. A Comprehensive View of the Characterisation of Exosomes. Sponsored by Wyatt Technology. Sep 25, 2018.
  3. Altadill, Tatiana., et al. (2016) Enabling Metabolomics Based Biomarker Discovery Studies Using Molecular Phenotyping of Exosome-Like Vesicles. PLoS One. 11(3):e0151339. PMCID: PMC4790956.
  4. Llorente A, et al. (2013) Molecular lipidomics of exosomes released by PC-3 prostate cancer cells. Biochim Biophys Acta. 1831(7):1302-9. PMID: 24046871.
  5. Schey, Kevin L., et al. (2016) Proteomics Characterization of Exosome Cargo. Methods. 87: 75–82.
  6. Schageman, Jeoffrey., et al. (2013) The Complete Exosome Workflow Solution: From Isolation to Characterization of RNA Cargo. Biomed Res Int. 2013:253957.

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