Creative Biostructure provides high-quality custom Mempro™ receptor-type kinase production services using cell-based expression system.
Mempro™ cell-based protein production system is the most commonly used for produce transmembrane proteins. Receptor-type kinases are a superfamily of membrane-bound and high-affinity receptor proteins that play a crucial role in signaling transduction, including inositol-phospholipid-calcium pathway and Ras pathway. Receptor tyrosine kinases and receptor protein serine/threonine kinases belong to receptor-type kinases family. A tyrosine kinase can phosphorylate tyrosine residues and a serine-threonine kinase serine or threonine residues. It has been reported that the structure of receptor-type kinase cantains a transmembrane domain, an extracellular ligand-binding domain and a cytosolic tail.
Figure 1. The structure of Receptor tyrosine kinase EphA1.
Creative Biostructure has extensive experence in native activity of receptor-type kinase production in cell-based protein expression system, we can provide various strategies for Mempro™ cell-based protein production, including:
Escherichia coli is the most commonly used bacterial host for the production of receptor-type kinases. Yeast cell is a perfect eukaryotic host because of its features, including single cell, fast growth rate, inexpensive media, and high cell density. Insect cell (such as Sf9, Sf21 and High Five) is also a major system for receptor-type kinase expression. Mammalian cell presents another eukaryotic system for receptor-type kinase expression, which provides nearly native-like cell environment. Mammalian cell lines derived from COS, CHO, BHK-21, HEK293, HeLa and GH3 are popularly used for integral membrane protein expression.
With the Mempro™ cell-based protein production platform, Creative Biostructure provides expressing, isolating, purifying and crystallizing receptor-type kinases to facilitate the study of their biological functions.
We provide other various Mempro™ membrane protein production services. Please feel free to contact us for a detailed quote.
References:C. Trometer, and P. Falson (2010). Mammalian membrane protein expression in baculovirus-infected insect cells. Methods Mol. Biol., 601: 105-117.J. Petschnigg, et al. (2011). Using yeast as a model to study membrane proteins. Curr. Opin. Nephrol. Hypertens., 20(4): 425-432.M. A. Lemmon and J. Schlessinger (2010). Cell signaling by receptor-tyrosine kinases. Cell, 141(7): 1117-1134.M. Shibuya, et al. (1990). Nucleotide sequence and expression of a novel human receptor-type tyrosine kinase gene (flt) closely related to the fms family. Oncogene, 5(4): 519-524.Receptor tyrosine kinase. (https://en.wikipedia.org/wiki/Receptor_tyrosine_kinase). S. Schlegel, et al. (2013). Bacterial-based membrane protein production. Biochim. Biophys. Acta., 1843(8): 1739-1749.T. Lai, et al. (2013). Advances in Mammalian cell line development technologies for recombinant protein production. Pharmaceuticals, 6(5): 579-603.