Based on the comprehensive membrane protein production platform establised through years of experience, experters from Creative Biostructure can offer custom Mempro™ single-helix ATPase regulators production services using cell-based expression system.
Mempro™ cell-based protein production system is the most widely used for membrane proteins production. Single-helix ATPase regulators are a large group of bitopic proteins with alpha-helical transmembrane anchors and regulate ATPases. It is well known that alpha-helices and beta-barrels are the main components of the transmembrane domains in transmembrane proteins. Single-helix ATPase regulators can be classified two families, including calcium ATPase regulators and FXYD regulators. Calcium ATPase regulators locate in endoplasm reticulum (ER) and regulate calcium homeostasis. Members of calcium ATPase regulators family include phospholamban, sarcolipin and cytoplasmic helix. FXYD regulators locate in the plasma membrane of eukaryo, members of FXYD regulators family are tissue-specific modulators of Na, K-ATPase. FXYD1 mainly increases the apparent affinity for intracellular Na+ of Na, K-ATPase. FXYD2 and FXYD4 can oppositely regulate the apparent affinity for Na+ of Na, K-ATPase.
Figure 1. The structural model of Phospholamban. (OPM Database)
Creative Biostructure can produce high-quality single-helix ATPase regulators, we can employ various strategies for Mempro™ cell-based protein production, including:
Escherichia coli (E. coli) is the powerful bacterial host for the production of single-helix ATPase regulators. Yeast cells (such as saccharomyces cerevisiae and pichia pastoris) system, combines prokaryotic as well as eukaryotic characteristics. It is a perfect eukaryotic host due to single cells, fast growth rates, inexpensive media, high cell densities. Insect cells (such as Sf9, Sf21 and High Five) are also the major system for single-helix ATPase regulators expression, which are developed from transfected insect cells in combination with vectors derived from the baculovirus species AcMNPV. Mammalian cells present another eukaryotic system for single-helix ATPase regulators expression, which provide near-native cell environment. Cell lines derived from COS, CHO, BHK-21, HEK293, HeLa and GH3 can be commonly used for transmembrane protein expression.
With the Mempro™ cell-based protein production platform, Creative Biostructure is capable of expressing, isolating, purifying and crystallizing single-helix ATPase regulators to facilitate the study of their physiological functions.
We provide other various Mempro™ membrane protein production services. Please feel free to contact us for a detailed quote.
References:D. H. MacLennan and E. G. Kranias. (2003) Phospholamban: a crucial regulator of cardiac contractility. Nat. Rev. Mol. Cell Biol., 4(7): 566-77.F. Junge, et al. (2008) Large-scale production of functional membrane proteins. Cellular Mol. Life Sci., 65 (11): 1729-1755.J. Petschnigg, et al. (2011). Using yeast as a model to study membrane proteins. Curr. Opin. Nephrol. Hypertens., 20(4): 425-432.K. Geering (2005). Function of FXYD proteins, regulators of Na, K-ATPase. J. Bioenerg. Biomembr., 37(6): 387-92. S. Schlegel, et al. (2013). Bacterial-based membrane protein production. Biochim. Biophys. Acta., 1843(8): 1739-1749.