Creative Biostructure has focus on membrane proteins research for years, and has established an advanced detergent-free membrane protein expression system. We can offer high quality tailored Mempro™ FAD/NAD(P)-binding domain production services for you.
FAD/NAD(P)-binding domain is a superfamily made up of 8 families: C-terminal domain of adrenodoxin reductase-like, FAD-linked reductases N-terminal domain, GDI-like N domain, Succinate dehydrogenase/fumarate reductase flavoprotein N-terminal domain, FAD/NAD-linked reductases, N-terminal and central domains, Thi4-like, GidA-like and HI0933 N-terminal domain-like. Rab escort protein 1 is one of the members of GDI-like N domain family, it is called as rab proteins geranylgeranyl transferase component A 1 as well. Rab escort protein 1 is an enzyme encoded by the CHM gene in humans. The Rab escort protein 1 ties up unprenylated Rab GTPases in the dimeric holoenzyme, whereafter, they display to the catalytic Rab GGTase subunit for the geranylgeranyl transfer reaction. It has reported that CHM gene interacts with RAB7A, RAB1A and RAB3A.
Figure. 1 The Ras-associated binding (Rab) protein – GTPase cycles. (European Journal of Pediatrics 2008)
Creative Biostructure has rich professional experience in FAD/NAD(P)-binding domain production using detergent-free membrane protein expression system, we can perform various strategies for Mempro™ detergent-free protein production, including:
Nanodiscs are soluble nanoscale phospholipid bilayers, which consist of membrane scaffold proteins (MSPs). The MSP used for generating Nanodiscs is based on the sequence of human serum apolipoprotein AI. Nanodiscs are being used to study isolation, purification and solubilization of membrane proteins.
Amphipols are projected to maintain transmembrane proteins solvable in aqueous solutions without detergent. They may be typically used in biophysics and biochemistry by the physicochemical properties of amphipols cut.
This method generates SMA lipid particles (SMALPs) in which a little disk of lipid bilayer revolved around by polymer encircles the membrane proteins. SMALP has a significant potential, because it makes membrane bilayers solubilized and can implant discs in detergents-free condition.
With our leading detergent-free expression system and experience of hundreds of successful cases, Creative Biostructure guarantees best services for FAD/NAD(P)-binding domain production.
Creative Biostructure provides other various Mempro™ membrane protein production services. Please feel free to contact us for a detailed quote.
References:
Cremers F P, Armstrong S A, Seabra M C, et al. REP-2, a Rab escort protein encoded by the choroideremia-like gene[J]. Journal of Biological Chemistry, 1994, 269(3): 2111-2117.
Rak A, Pylypenko O, Niculae A, et al. Crystallization and preliminary X-ray diffraction analysis of monoprenylated Rab7 GTPase in complex with Rab escort protein 1[J]. Journal of structural biology, 2003, 141(1): 93-95.
Eppink M H M, Van Berkel W J H, Schreuder H A. Identification of a novel conserved sequence motif in flavoprotein hydroxylases with a putative dual function in FAD/NAD (P) H binding[J]. Protein Science, 1997, 6(11): 2454-2458.
Alexandrov K, Simon I, Iakovenko A, et al. Moderate discrimination of REP‐1 between Rab7⋅ GDP and Rab7⋅ GTP arises from a difference of an order of magnitude in dissociation rates[J]. FEBS letters, 1998, 425(3): 460-464.
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