Creative Biostructure can provide unmatched custom Mempro™ peptide fragments of transmembrane protein production services using detergent-free expression system. We can provide custom services for synthesis, purification, sequencing and characterization of peptide fragments from transmembrane proteins.
Transmembrane protein (TM) is a integral membrane protein spanning the entirety of the cell membrane, whic is embedded between the phospholipids. Transmembrane protein can be classified as alpha helical and beta barrel, depending on how the protein chain (also called peptide segments) is organized. Owing to the characteristics of transmembrane protein, the accurate structural information of thes proteins is very difficut to be obtained. Peptide structural analysis is crucial for the evaluation of biochips and biodevices. Creative Biostructure can supply optimal synthisis and expression of peptide fragments of transmembrane proteins to facilitate the study of transmembrane segments.
Figure 1. Peptide mimics of an anti-inflammatory protein. (Front. Bioeng. Biotechnol., 2016)
Creative Biostructure has great expertise in high-yield peptide fragments of transmembrane prtoein production using detergent-free membrane protein expression systmes, we can carry out various strategies for Mempro™ detergent-free peptide fragments of transmembrane protein production, including:
Nanodiscs are the self-assembly nanotechnology to stabilize membrane proteins removed from the membrane by membrane scaffold proteins (MSPs) in no need of detergent. Creative Biostructure can provide combination of synthesis of peptide fragments into pre-assembly nanodiscs for the study of characterization.
The amphipathic amphipols have the ability to “trap” around transmembrane regions of proteins, allowing them to stay folded. Creative Biostructure can apply A8-35 to solubilize synthesized peptide fragments.
The SMALPs are self-assembled by the simple addition of the SMA co-polymer. At neutral or alkaline pH, a disc-like structure assembles itself, encapsulating peptide fragments in a form amenable to be purified.
These novel detergent-free purification approaches for peptide fragments of transmembrane protein production can be obtained easily, and facilitate more comprehensively structural and functional analysis.
Creative Biostructure can provide various custom Mempro™ membrane protein production services. Please feel free to contact us for a detailed quote.
References:A. Groß, et al. (2016). Synthetic Peptides as Protein Mimics. Front. Bioeng. Biotechnol., http://dx.doi.org/10.3389/fbioe.2015.00211C. Jie, et al. (2007). Cyclization reaction of peptide fragment ions during multistage collisionally activated decomposition: an inducement to lose internal amino-acid residues. J. Am. Soc. Mass Spectrom., 18(4): 663–678.C. Tribet, et al. (1996). Amphipols: Polymers that keep membrane proteins soluble in aqueous solutions. Proc Natl Acad Sci U. S. A. 93: 15047-15050.M. Jamshad, et al. (2011). Surfactant-free purification of membrane proteins with intact native membrane environment. Biochem. Soc. Trans. 39: 813-818.