Based on the unparalleled detergent-free expression system established for years, Creative Biostructure can offer tailored Mempro™ tetratrico-peptide repeat superfamily production services. Scientists from Creative Biostructure are confident in providing best services for you.
Tetratrico-peptide repeat superfamily is composed of three families: Tetratricopeptide repeat family contained Peroxisomal targeting signal 1 receptor, Peroxisomal targeting signal 1 receptor complex with sterol carrier protein 2 and Peroxisome targeting signal 1 receptor PEX5, SusD family contained SusD superfamily protein and SNAP family contained Vesicular transport protein sec17. Sec17 is made up of a C-terminal alpha-helical bunch and a N-terminal twisted sheet of alpha-helical hairpins. The N-terminal sheet is partly similar to the tetratricopeptide repeats from protein phosphatase 5 but has an unlike overall twist. Besides, the structural features of clathrin heavy chain repeats and HEAT are same as Sec17. In addition, the gene encoding PEX5 also named as LdPEX5, are overexpressed and purified in Escherichia coli, which is used for binding experiments and generation of antibodies.
Figure 1. The overall structure of Vesicular transport protein sec17. (Molecular Cell, 1999)
Creative Biostructure has rich professional experience in high quality tetratrico-peptide repeat superfamily production using detergent-free membrane protein expression system, we can perform various strategies for Mempro™ detergent-free protein production, including:
Nanodiscs can magically make membrane proteins stabilized, due to their structural characteristics, membrane proteins in aqueous solutions are also solubled by nanodisc. In contrast with bicelles, detergent micelles and liposomes, a more native environment where proteins remain monodisperse and active can be offered.
Amphipols (APols) are projected to maintain transmembrane proteins solvable in aqueous solutions without detergent, in the essence, it’s a family of amphipathic polymers.
As a biochemical approach, SMALP has a significant potential, because it makes membrane bilayers solubilized and can implant discs in detergents-free conditions. The comprehend of membrane proteins can possibly be advanced by biophysical studies of disc-structure.
With our leading detergent-free expression system and experience for hundreds of successful cases, Creative Biostructure guarantees best services in tetratrico peptide repeat superfamily production for customers.
Creative Biostructure provides other various Mempro™ membrane protein production services. Please feel free to contact us for a detailed quote.
References:
Gindhart J G, Goldstein L S B. Tetratrico peptide repeats are present in the kinesin light chain[J]. Trends in biochemical sciences, 1996, 21(2): 52-53.
Zhang H, Grishin N V. The α‐subunit of protein prenyltransferases is a member of the tetratricopeptide repeat family[J]. Protein science, 1999, 8(8): 1658-1667.
Callahan M A, Handley M A, Lee Y H, et al. Functional interaction of human immunodeficiency virus type 1 Vpu and Gag with a novel member of the tetratricopeptide repeat protein family[J]. Journal of virology, 1998, 72(6): 5189-5197.
Marty C, Browning D D, Richard D Y. Identification of tetratricopeptide repeat 1 as an adaptor protein that interacts with heterotrimeric G proteins and the small GTPase Ras[J]. Molecular and cellular biology, 2003, 23(11): 3847-3858.
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