Creative Biostructure is a specialized manufacturer of membrane protein production based on our advanced technology and years of experience. Creative Biostructure can offer tailored Mempro™ Transcriptional factor tubby production services using detergent-free expression system.
Transcription factors are a type of proteins which can bind to specific DNA sequences in molecular biology. The genetic information transcription rate from DNA to messenger RNA is regulated by transcription factor. Tubby protein is one of the transcription factors encoded by the TUB gene. An alpha helix encircled by a beta barrel is in the central pore of Tubby proteins and they can bind to phosphatidylinositol which is typically regarded as a small signaling molecule localized to the membrane. It has indicated that tubby proteins are potential signaling factors involved in G-protein activity. Several diseases are caused by the mutations of tubby proteins, such as retinal degeneration, obesity, and hearing loss.
Figure 1. Proposed molecular functions of TULPs. (Journal of Cell Science 2002)
Creative Biostructure has rich professional experience in high quality transcriptional factor tubby production using detergent-free membrane protein expression system, we can perform several strategies for Mempro™ detergent-free protein production, including:
Nanodisc is self-assembling discoidal nanoparticles which consists of amphiphilic α-helical scaffold proteins or peptides warped around the circumference of a lipid bilayer in a belt-like way. It’s currently accepted that nanodisc stands for the simplest models of high density lipoprotein(HDL) particles.
Amphipols are absolutely practicable tools for handling membrane proteins in detergent-free water solutions, and essentially they are a new class of surfactants. Amphipols were synthesized by forming amide bonds between octylamine and, sometimes, isopropylamine.
This technique generates SMA lipid particles (SMALPs) in which a little disk of lipid bilayer surrounded by polymer encircles the membrane proteins. Besides, the use of SMALPs can make detergent-free isolation of membrane proteins and retention of their crude lipid environment available.
With our advanced detergent-free expression system and experience for years, Creative Biostructure guarantees unparalleled services in Transcriptional factor tubby production.
Creative Biostructure provides other various Mempro™ membrane protein production services. Please feel free to contact us for a detailed quote.
References: Boggon T J, Shan W S, Santagata S, et al. Implication of tubby proteins as transcription factors by structure-based functional analysis[J]. Science, 1999, 286(5447): 2119-2125.Kleyn P W, Fan W, Kovats S G, et al. Identification and characterization of the mouse obesity gene tubby: a member of a novel gene family[J]. Cell, 1996, 85(2): 281-290.Latchman D S. Transcription factors: an overview[J]. The international journal of biochemistry & cell biology, 1997, 29(12): 1305-1312.Roeder R G. The role of general initiation factors in transcription by RNA polymerase II[J]. Trends in biochemical sciences, 1996, 21(9): 327-335.