Creative Biostructure has developed an excellent Mempro™ membrane protein production platform through many years of experience. Our first-class custom Mempro™ annexin production services are based on the innovative plant-based expression system.
Annexins are a well-known family of membrane binding proteins, which are Ca2+-regulated phospholipid binding proteins. Members of annexin family possess four conserved structural elements termed annexin repeats composed of around 70 amino acid residues. Annexin repeat is highly α-helical and slightly curved disc that has a surface containing the Ca2+- and membrane-binding sites. It has been reported that annexins play important roles in vesicle transport (such as exocytosis and endocytosis) and calcium ion channel formation. In addition, annexins can function as membrane scaffolding proteins to anchor other proteins to the cell membrane.
Figure 1. The structural model of Annexin A5.
Creative Biostructure can provide you the best membrane protein production services with animal-free, low endotoxin, and low protease activity. Recombinant membrane proteins expressed by our plant system are free of animal components, serum, endotoxins and antibiotics as well as human or animal infectious agents or other endogenous mammalian contamination. Notably, animal-free recombinant membrane proteins are particularly crucial for customers concerned with experimental variables caused by trace animal components or mammalian pathogens.
Creative Biostructure is highly experienced in providing membrane protein production services based on various types of plants, such as Nicotiana benthamiana (tobacco), Medicago rativa (alfalfa), Arabidopsis thaliana (A. thaliana), potato, maize, barley and lettuce. And plant system holds many advantages in membrane production services:
- Cost-effective
- Easy storage and distribution
- Environment friendly
- Free of infectious or toxic contaminants
- High scale-up capability
With the Mempro™ plant-based protein production platform, Creative Biostructure is capable of expressing, isolating, purifying and crystallizing annexins to facilitate the study of their physiological functions. Misfolding, aggregation, inactivity, poor stability and solubility, etc. are the common difficulties encountered in cell-based expression system. Strikingly, these difficulties can be overcomed in the renewed plant-based expression system.
Creative Biostructure can also provide Mempro™ plant-based virus-like particles (VLPs) production services, Mempro™ animal-free recombinant protein production services and Mempro™ membrane protein production services. Please feel free to contact us for a detailed quote.
References:
A. Wiktorek-Smagur, et al. (2012). Green way of biomedicine – how to force plants to produce new important proteins. Transgenic Plants-Advances and Limitations, Chapter 3. doi: 10.5772/1409.
F. Junge, et al. (2008) Large-scale production of functional membrane proteins. Cellular Mol. Life Sci., 65 (11): 1729-1755.
U. Rescher and V. Gerke (2004). Annexins - unique membrane binding proteins with diverse functions. J. Cell Sci., 117: 2631-2639.
V. Gerke and S. E. Moss (2002). Annexins: from structure to function. Physiol. Rev., 82(2): 331-371.
