Creative Biostructure has established the comprehensive membrane protein production platform through many years of experience, our experts can provide customized Mempro™ immunoglobulin superfamily protein production services based on plant-derived expression system.
Immunoglobulins are known as antibodies, which can be involved in the recognition, binding, or adhesion processes of cells, such as CD4, CD8, T cell receptor (TCR), and human leukocyte antigen (HLA). The immunoglobulin superfamily (IgSF) is a large class of cell surface and soluble proteins, Members of IgSF possess a structural domain, termed immunoglobulin domain (Ig domain), which contains about 70-110 amino acids. Ig domain can be found in numerous proteins, such as cell surface antigen receptors, co-receptors and co-stimulatory molecules of the immune system, cell adhesion molecules, cytokine receptors and intracellular muscle proteins. In addition, IgSF proteins play important roles in the immune system.
Figure 1. Schematic diagram of immunoglobulin superfamily proteins that are found in neurons. (MBInfo)
Mempro™ plant-based protein production platform can produce animal-free, low endotoxin, and low protease activity membrane proteins. Animal-free recombinant membrane proteins are particularly crucial for customers concerned with experimental variables caused by trace animal components or mammalian pathogens. Recombinant membrane proteins expressed by this platform are free of animal components, serum, endotoxins and antibiotics as well as human or animal infectious agents or other endogenous mammalian contamination. Additionally, post-translational modification such as glycosylation and disulfide bonds can be introduced in plant system.
Creative Biostructure can provide membrane protein production services based on various types of host plants, including Nicotiana benthamiana (tobacco), Medicago rativa (alfalfa), Arabidopsis thaliana (A. thaliana), potato, maize, barley and lettuce. The significant advantages of plant-based membrane protein production are listed below:
With the Mempro™ plant-based protein production platform, Creative Biostructure is capable of expressing, isolating, purifying and crystallizing IgSF proteins to facilitate the study of their biological functions. Misfolding, aggregation, inactivity, poor stability, high endotoxin and solubility, etc. are the common difficulties encountered in cell-based expression system. Strikingly, these difficulties can be overcomed in the renewed plant-based expression system.
Creative Biostructure can also provide Mempro™ plant-based virus-like particles (VLPs) production services, Mempro™ animal-free recombinant protein production services and Mempro™ membrane protein production services. Please feel free to contact us for a detailed quote.
References:A. Wiktorek-Smagur, et al. (2012). Green way of biomedicine – how to force plants to produce new important proteins. Transgenic Plants-Advances and Limitations, Chapter 3. doi: 10.5772/1409.F. Junge, et al. (2008) Large-scale production of functional membrane proteins. Cellular Mol. Life Sci., 65 (11): 1729-1755. K． Natarajan, et al. (2015). Immunoglobulin superfamily. eLS, doi: 10.1002/9780470015902.a0000926.pub2 S. Schlegel, et al. (2013). Bacterial-based membrane protein production. Biochim. Biophys. Acta., 1843(8): 1739-1749. Y. Shimono, et al. (2012). Immunoglobulin superfamily receptors and adherens junctions. Subcell Biochem., 60: 137-170.