Creative Biostructure has developed the first-class membrane protein production platform through many years of experience, we can provide customized Mempro™ major instrinsic protein (MIP)/formate-nitrite transporter (FNT) superfamily protein production services using plant-based expression system.
Members of MIP superfamily belong to transmembrane protein channels (six transmembrane helices, TM1-TM6; and two half-helices, LB and LE) that facilitate water and solute permeability across cell membranes and are found in eubacteria, archaea, fungi, plants and animals. Aquaporin (AQP) and aquaglyceroporin channels are the primary members of the MIP superfamily. AQPs are expressed in a wide range of tissues, often spatially located within a certain region of the cell. Members of FNT superfamily can function as channels monovalent anions, and which contain a structural domain termed the aquaporin helical fold. The Transport Classification Database (TCDB) shows that the FNT superfamily in included in the MIP superfamily.
Figure 1. The structural model of aquaglyceroporin. (OPM Database)
Mempro™ plant-based protein production platform has a lot of advantages, such as animal-free, low endotoxin, and low protease activity. Membrane proteins expressed by our plant system are free of animal components, serum, endotoxins and antibiotics as well as human or animal infectious agents or other endogenous mammalian contamination. As we known, animal-free recombinant membrane proteins are particularly crucial for customers concerned with experimental variables caused by trace animal components or mammalian pathogens. In addition, post-translational modification such as glycosylation and disulfide bonds can be introduced in plant system.
Creative Biostructure can offer membrane protein production services using various types of host plants, such as Nicotiana benthamiana (tobacco), Medicago rativa (alfalfa), Arabidopsis thaliana (A. thaliana), potato, maize, barley and lettuce, etc. We can provide two different strategies for recombinant membrane protein production in plants: transient expression and stable transformation.
With the Mempro™ plant-based protein production platform, Creative Biostructure is capable of expressing, isolating, purifying and crystallizing MIP/FNT superfamily proteins to facilitate the study of their structural and biological functions.
Creative Biostructure can also provide Mempro™ plant-based virus-like particles (VLPs) production services, Mempro™ animal-free recombinant protein production services and Mempro™ membrane protein production services. Please feel free to contact us for a detailed quote.
References:A. Wiktorek-Smagur, et al. (2012). Green way of biomedicine – how to force plants to produce new important proteins. Transgenic Plants-Advances and Limitations, Chapter 3. doi: 10.5772/1409.M. Mukherjee, et al. (2016). Functional diversity within the fnt superfamily of anion channels: phylogenetics & molecular dynamics studies. Biophysical J., 110(3): p117a.N. Yan (2015). Structural biology of the major facilitator superfamily transporters. Annu. Rev. Biophys., 44: 257-283.R. K. Verma, et al. (2015). Major intrinsic protein superfamily: channels with unique structural features and diverse selectivity filters. Methods Enzymol., 557: 485-520.