With years of experience in the field of recombinant membrane protein production, Creative Biostructure now provides custom Mempro™ protein kinase superfamily production services using virus-like particles (VLPs) with our most advanced technology.
Protein kinases modify other proteins by catalyzing the proteins' reversible phosphorylation, and act as critical regulators of nearly all of cell functions and processes. By adding the phosphate group reversibly to the substrate proteins, they regulate the activity of enzymes, the location and overall function of various proteins. Protein kinases make up one of the largest superfamilies of homologous proteins. There are more than 500 protein kinases within human cells, 478 of them belong to a single superfamily with their catalytic domain are related in sequence.
Figure 1. The schematic structure of Calcium/calmodulin-dependent protein kinase type IV. (OPM Database)
Creative Biostructure now can provide high-quality and high-yield Mempro™ custom protein kinases superfamily production services using virus-like particles. We are able to retain the native structure and orientation of target protein with our most advanced VLPs-lipoparticles technique. Various production systems are available in Creative Biostructure to meet different experimental requirements of our customers, including E. coli, yeasts, insect cells, green plants (e.g. tobacco), and mammalian cells.
VLPs are self-assembled subunits that can be described as "empty viral capsomere". They are highly organized constructions that can mimic native virus, except that all the VLPs are non-infectious because of lacking viral genomes. VLPs have been used in various biological and clinical researches as powerful tools such as vaccine development, antigen delivery, recombinant membrane protein production, and heterologous epitope presentation. We can use several virus families to produce VLPs, including bacteriophages, Parvoviridae, Retroviridae, etc.
Creative Biostructure will develop the optimal production strategies in each individual case. Our science team will perform the co-expression of viral structural core protein and the target protein kinases in the chosen host cells. The recombinant viral structural core protein will proceed the self-assembly in the plasmid membrane of host cells to build VLPs and then bud-off from the plasmid membrane as lipoparticles (VLPs that that contain high concentration of specific membrane proteins within their construction). The expressed target protein kinase will be captured by lipoparticles during the formation of lipoparticles. We can harvest the specific lipoparticles at the surface of host cells along with the target protein kinase within its construction. The entire process is free from detergents and mechanical disruptions, so that the native structure and orientation of target inter-membrane proteins can be protected.
Creative Biostructure also provides a wide range of other Mempro™ membrane protein production service with various advanced techniques. Welcome to contact us for more details.
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