PNExo™ Exosome-Rose(PNE-FRO75)

Benefits of Rose: Active Compounds and Biological Functions

Rosa species are rich in diverse phytochemicals that contribute to their antioxidant, anti-inflammatory, and skin-supporting properties. Key bioactive compounds include:

• Essential Oils: Components like citronellol and geraniol reduce inflammation, relieve stress, and exhibit antimicrobial effects by disrupting microbial membranes.
• Flavonoids: Quercetin and kaempferol possess antioxidant and anti-inflammatory effects, promote cardiovascular health, and help regulate lipid metabolism.
• Triterpenes: Ursolic and oleanolic acids support immune activation, modulate blood sugar, and protect gastric mucosa.
• Polyphenols: Gallic and ellagic acids scavenge free radicals, inhibit melanin production, and help delay skin aging through anti-glycation mechanisms.
• Polysaccharides & Vitamin C: Found in rose hips, these enhance collagen synthesis, support immune responses, and aid detoxification.

What Are Rose-Derived Exosomes?

Rose-derived exosomes are nanoscale extracellular vesicles (30-200 nm) secreted by Rosa plant cells. Enclosed by a lipid bilayer, they are structurally and functionally similar to animal-derived exosomes, yet uniquely plant-based. These vesicles carry bioactive molecules such as proteins, lipids, and miRNAs, including over 200 distinct peptides and Let-7 family microRNAs, which have been linked to cellular regeneration and pigment regulation.

Rose exosomes fall into two main categories:

• Standard Rose Exosomes: Isolated directly from rose tissues, these exosomes exhibit low immunogenicity and are rich in plant-specific proteins and regulatory RNAs. They are involved in cell-to-cell communication and stress responses and may influence skin health via antioxidant and anti-inflammatory pathways.
• Rose Stem Cell Exosomes (RSCEs): Purified from the conditioned media of cultured rose stem cells using tangential flow filtration (TFF), RSCEs are a highly refined subclass with enhanced bioactivity. They feature higher concentrations of functional molecules such as Let-7 miRNAs and annexins. These exosomes have demonstrated increased stability and cellular compatibility and are considered more suitable for targeted applications.

Rose Stem Cell Exosomes vs. Conventional Rose Extracts

Global Sources of Exosome-Derived Products Used in Skin Rejuvenation. (Theodorakopoulou E, et al., 2024)

Potential Applications of Rose-Derived Exosomes

Ongoing research has revealed promising functional potential for rose-derived exosomes, particularly in skin-related formulations. Key areas of interest include:

• Skin Renewal and Repair: RSCEs have been shown to promote dermal fibroblast proliferation and accelerate wound closure in scratch assays, likely through miRNA-regulated collagen synthesis pathways. They exhibit no cytotoxicity at high concentrations, making them suitable for sensitive applications.
• Pigmentation Modulation: RSCEs can reduce melanin content in melanocytes in a dose-dependent manner by inhibiting tyrosinase activity and promoting melanosome autophagy. These effects support their inclusion in brightening and skin-tone evening formulations.
• Anti-Inflammatory and Barrier Support: By reducing the secretion of IL-6 in LPS-stimulated macrophages, RSCEs may help calm inflammation and restore skin barrier function, particularly in irritation-prone or reactive skin conditions.
• Bioactive Delivery Vehicles: Due to their plant origin, biocompatibility, and ability to cross biological barriers, rose-derived exosomes are being investigated as natural nanocarriers for delivering miRNAs or other active ingredients in skincare or nutraceutical systems.

While still under scientific exploration, rose-derived exosomes represent a next-generation botanical platform with broad potential in cosmetic science, regenerative skincare, and functional bio-delivery.

References

1. Won Y J, Lee E, Min S Y, et al. Biological function of exosome-like particles isolated from Rose (Rosa Damascena) stem cell culture supernatant. bioRxiv, 2023: 2023.10. 17.562840.
2. Lueangarun S, Cho B S, Tempark T. Rose stem cell-derived exosomes for hair regeneration enhancement via noninvasive electroporation in androgenetic alopecia. Journal of Cosmetic Dermatology. 2024, 23(11).
3. Lueangarun S, Cho B S, Tempark T. Topical moisturizer with rose stem cell-derived exosomes (RSCEs) for recalcitrant seborrheic dermatitis: A case report with 6 months of follow-up. Journal of Cosmetic Dermatology. 2024, 23(10): 3128-3132.
4. Theodorakopoulou E, Aguilera S B, Duncan D I. A new therapeutic approach with rose stem-cell-derived exosomes and non-thermal microneedling for the treatment of facial pigmentation. Aesthetic Surgery Journal Open Forum. US: Oxford University Press, 2024, 6: ojae060.
5. Majewska L, Dorosz K, Kijowski J. Efficacy of Rose Stem Cell-Derived Exosomes (RSCEs) in Skin Treatment: From Healing to Hyperpigmentation Management: Case Series and Review. Journal of Cosmetic Dermatology. 2025, 24(1): e16776.

Case Study 1: RSCEs Combined with Microneedling for Facial Pigmentation (Theodorakopoulou E, 2024)

A recent clinical study assessed the use of rose stem cell-derived exosomes (RSCEs) with nonthermal microneedling in 12 women with melasma, solar lentigines, or postinflammatory hyperpigmentation. Participants received three topical treatments at three-week intervals, followed by red LED light therapy.

By Week 12, superficial pigmentation decreased by 12.95 percent, deep pigmentation by 15.9 percent, skin redness by 7.34 percent, and wrinkles by 6.34 percent.

The RSCEs, purified using tangential flow filtration and delivered through microneedling, showed depigmenting and anti-inflammatory effects. In vitro findings suggest RSCEs reduce melanin in melanocytes and lower IL-6 in macrophages.

This study offers early clinical support for RSCEs as a safe, plant-based approach to improving skin tone, texture, and pigmentation without adverse effects.

Figure 1. Imaging Assessment of Deep Pigmentation Improvement After RSCE Treatment. Imaging analysis of a 44-year-old female patient showing facial pigmentation changes. (A) Initial distribution of deep pigmentation before RSCE treatment. (B-D) Progressive improvement of pigmentation at Week 3, Week 6, and Week 12 post-treatment, respectively.

Figure 2. Imaging Assessment of Sensitive Skin Improvement Following RSCE Treatment. Imaging analysis of a 50-year-old female patient with sensitive skin prone to rosacea, inflammation, and telangiectasia. (A) Baseline image showing the intensity and distribution of skin sensitivity. (B-D) Progressive reduction in skin sensitivity at Week 3, Week 6, and Week 12 after treatment.

Case Study 2: Rose Stem Cell-Derived Exosomes in Hair Regeneration (Lueangarun S, 2024)

A clinical report examined the use of RSCEs for androgenetic alopecia (AGA). A 54-year-old male with advanced hair loss (Hamilton-Norwood Scale V) received 12 sessions of topical RSCEs delivered via noninvasive electroporation over three months.

The treatment involved 20 mg of lyophilized RSCEs in 5 mL solution, applied using electroporation to enhance transdermal absorption. Visible improvements in hair density and thickness were noted after the third session, with continued progress through the 6th and 12th treatments. Results remained stable three months post-treatment. Dermoscopic imaging confirmed increased follicle density and hair shaft thickness, with no reported adverse effects.

The RSCEs, purified via tangential flow filtration, contained over 200 proteins and miRNAs such as Let-7 and miR-1246, which regulate inflammation and promote dermal papilla cell growth. Unlike crude extracts, RSCEs were non-toxic to hair follicle cells and effectively stimulated their proliferation.

This case highlights the potential of RSCEs as a safe, noninvasive option for hair restoration, though larger clinical studies are needed to validate these findings.

Figure 1. Clinical Outcomes of Exosome Therapy for Androgenetic Alopecia. (A) Baseline showing diffuse scalp hair thinning and frontal-temporal hairline recession. (B) After the 3rd treatment session: visible improvements in hair density, reduced shedding, and increased thickness. (C-D) Continued improvement seen after the 6th and 12th sessions with exosome therapy and electroporation. (E) Sustained hair regrowth and density enhancement observed 3 months post-treatment.

Figure 2. Dermoscopic Evaluation of Mid-Scalp Area During Exosome Therapy. Dermoscopic images of the mid-scalp region taken at three time points: (A) Baseline before treatment, (B) After the 6th session, (C) After the 12th session of exosome electroporation therapy. A nevus and cherry angioma serve as consistent reference markers. No serious adverse events were reported, and the treatment was well-tolerated.

References

1. Theodorakopoulou E, Aguilera S B, Duncan D I. A new therapeutic approach with rose stem-cell-derived exosomes and non-thermal microneedling for the treatment of facial pigmentation. Aesthetic Surgery Journal Open Forum. US: Oxford University Press, 2024, 6: ojae060.
2. Lueangarun S, Cho B S, Tempark T. Rose stem cell-derived exosomes for hair regeneration enhancement via noninvasive electroporation in androgenetic alopecia. Journal of Cosmetic Dermatology. 2024, 23(11).

• Is there batch-to-batch consistency in terms of particle count and biological activity?

  Each lot is quality-controlled for particle concentration (>1×10¹⁰ particles/mL), size distribution (via NTA), and key bioactivity markers. CoAs and batch reports are available upon request.

• Can PNExo™ Rose Exosomes be labeled or fluorescently tagged for in vitro tracking?

  While the base product is unlabeled, we offer custom conjugation or co-packaging with fluorescent dyes suitable for exosome uptake or biodistribution studies.

• Is the product animal-free and suitable for vegan or clean-label formulations?

  Yes, the PNExo™ Exosome-Rose is fully plant-derived with no animal-origin inputs, suitable for plant-based or vegan formulations.

• Can the exosomes be used in cell culture experiments or functional assays?

  Yes, the exosomes are suitable for in vitro applications including fibroblast proliferation, antioxidant activity, melanin inhibition assays, and inflammation models. Please consult with our technical team for recommended working concentrations.

• Can the product be scaled for pilot production with GMP compliance?

  Yes, Creative Biostructure offers custom scale-up services including GMP-grade manufacturing and CDMO support for formulation or preclinical studies.

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