MAD2-p3, Mad2-p31(comet) complex

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MAD2 with p31 Protein Crystal(CBCRY03)

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Product Summary
Name	MAD2 with p31 Protein Crystal Download Datasheet

Background
Background	The crystal structure of the Mad2-p31(comet) complex is reported. The C-terminal region of Mad2 that undergoes rearrangement in different Mad2 conformers is a major structural determinant for p31(comet) binding, explaining the specificity of p31(comet) toward Mad1- or Cdc20-bound Mad2. p31(comet) adopts a fold strikingly similar to that of Mad2 and binds at the dimerization interface of Mad2. Thus, p31(comet) exploits the two-state behavior of Mad2 to block its activation by acting as an "anti-Mad2."

Background

The crystal structure of the Mad2-p31(comet) complex is reported. The C-terminal region of Mad2 that undergoes rearrangement in different Mad2 conformers is a major structural determinant for p31(comet) binding, explaining the specificity of p31(comet) toward Mad1- or Cdc20-bound Mad2. p31(comet) adopts a fold strikingly similar to that of Mad2 and binds at the dimerization interface of Mad2. Thus, p31(comet) exploits the two-state behavior of Mad2 to block its activation by acting as an "anti-Mad2."

Molecular Description
Protein Classification	cell cycle
Structure Weight	105471.24 Da
Polymer	1
Molecule	Mitotic spindle assembly checkpoint protein MAD2A
Chain Length	210 amino acids
Polymer	2
Molecule	MAD2L1-binding protein
Chain Length	268 amino acids
Polymer	3
Molecule	peptide
Chain Length	12 amino acids

Crystal Description
PDB ID	2QYF
Source	E.coli
Method	X-Ray Diffraction
Resolution	2.30 Å

Gene Information
Gene Name	MAD2L1
Synonyms	HSMAD2; MAD2; MAD2 (mitotic arrest deficient, yeast, homolog)-like 1; MAD2-like 1; MAD2-like protein 1; mitotic arrest deficient, yeast, homolog-like 1
UniProt ID	Q13257
Gene ID	4085
Chromosome Location	4q27
Function	protein binding
Gene Name	MAD2L1BP
Synonyms	MAD2L1 binding protein; CMT2, KIAA0110, MGC11282, RP1-261G23.6; OTTHUMP00000016496; caught by MAD2 protein; KIAA0110; dJ261G23.1
UniProt ID	Q15013
Gene ID	9587
Chromosome Location	6p21.1
Function	protein binding

Reference
Reference	Yang, M., Li, B., Tomchick, D.R., Machius, M., Rizo, J., Yu, H., Luo, X. p31comet blocks Mad2 activation through structural mimicry.Cell(Cambridge,Mass.)2007; 131: 744-755

MAD2 with p31 Protein Crystal

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MAD2 with p31 Protein Crystal(CBCRY03)

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