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MAD2 with p31 Protein Crystal(CBCRY03)

Product Summary
Name MAD2 with p31 Protein Crystal Download Datasheet
Background
Background The crystal structure of the Mad2-p31(comet) complex is reported. The C-terminal region of Mad2 that undergoes rearrangement in different Mad2 conformers is a major structural determinant for p31(comet) binding, explaining the specificity of p31(comet) toward Mad1- or Cdc20-bound Mad2. p31(comet) adopts a fold strikingly similar to that of Mad2 and binds at the dimerization interface of Mad2. Thus, p31(comet) exploits the two-state behavior of Mad2 to block its activation by acting as an "anti-Mad2."
Molecular Description
Protein Classificationcell cycle
Structure Weight105471.24 Da
Polymer1
MoleculeMitotic spindle assembly checkpoint protein MAD2A
Chain Length210 amino acids
Polymer2
MoleculeMAD2L1-binding protein
Chain Length268 amino acids
Polymer3
Moleculepeptide
Chain Length12 amino acids
Crystal Description
PDB ID2QYF
SourceE.coli
MethodX-Ray Diffraction
Resolution2.30 Å
Gene Information
Gene NameMAD2L1
SynonymsHSMAD2; MAD2; MAD2 (mitotic arrest deficient, yeast, homolog)-like 1; MAD2-like 1; MAD2-like protein 1; mitotic arrest deficient, yeast, homolog-like 1
UniProt IDQ13257
Gene ID4085
Chromosome Location4q27
Functionprotein binding
Gene NameMAD2L1BP
SynonymsMAD2L1 binding protein; CMT2, KIAA0110, MGC11282, RP1-261G23.6; OTTHUMP00000016496; caught by MAD2 protein; KIAA0110; dJ261G23.1
UniProt IDQ15013
Gene ID9587
Chromosome Location6p21.1
Functionprotein binding
Reference
Reference Yang, M., Li, B., Tomchick, D.R., Machius, M., Rizo, J., Yu, H., Luo, X. p31comet blocks Mad2 activation through structural mimicry.Cell(Cambridge,Mass.)2007; 131: 744-755

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