Creative Biostructure has years of experience in the comprehensive membrane protein production, scientists from Creative Biostructure can offer customized Mempro™ Ion Channels Superfamily production services using detergent-free protein expression system.
Ion channels are pore-forming membrane proteins which have numerous crucial functions including regulating cell volume, setting up a resting membrane potential, forming action potentials and other electrical signals and so on. Taking several members of the ClC family as an example, they are excellent in plant vacuoles and in endosomes. Such variety enhances the notion that ion channels and ion transporters (carriers) are formally and, in some cases, structurally related membrane proteins. A few families of ion channels are controlled by extracellular ligands, and the cysteine-loop channels of rapid chemical synapses is one of the members outstanding. The cysteine-loop channels are specialized as receptors for the serotonin (5-HT), glycine (gly), acetylcholine (ACh), which are all chemical neurotransmitter. They not only offer inhibitory but also excitatory synaptic transmission.
Figure 1. Principle of ion-channel-coupled receptors (ICCRs). (Nature Nanotechnology, 2008)
Creative Biostructure has rich professional experience in high quality ion channels superfamily production based on detergent-free membrane protein expression system, we can perform various strategies for Mempro™ detergent-free protein production, including:
Nanodiscs can be stabilized in solution through the assist of amphipathic helical scaffold protein, and also they are advanced tools to learn interacting companions of membrane embedded molecules. They provide an inartificial phospholipid bilayer environment for target membrane proteins in the solubilized state.
Amphipols (APols) are a new class of surfactants which enable to keep membrane proteins soluble in water without common surfactants. They are highly usable tools for handling membrane proteins in detergent-free water solutions and generally used for biophysical researches.
SMALPs are parceled by the amphipathic styrene maleic acid polymer (SMA). SMA copolymer consists of a polymer which is mixed by maleic anhydride and styrene in all kinds of proportions. Using SMALPs can make detergent-free isolation of membrane proteins and retention of their crude lipid environment available.
With our leading detergent-free expression system and experience for hundreds of successful cases, Creative Biostructure guarantees our customers unparalleled service in Ion channels superfamily production.
Creative Biostructure provides other various Mempro™ membrane protein production services. Please feel free to contact us for a detailed quote.
References: Bayburt, T. H., & Sligar, S. G. (2010). Membrane protein assembly into Nanodiscs. FEBS letters, 584(9), 1721-1727. Postis, V., Rawson, S., Mitchell, J. K., Lee, S. C., Parslow, R. A., Dafforn, T. R., . . . Muench, S. P. (2015). The use of SMALPs as a novel membrane protein scaffold for structure study by negative stain electron microscopy. Biochimica et Biophysica Acta (BBA)-Biomembranes, 1848(2), 496-501. Yan, N. (2015). Structural biology of the major facilitator superfamily transporters. Annual review of biophysics, 44, 257-283. Dhanasekaran, N., & Reddy, E. P. (1998). Signaling by dual specificity kinases. Oncogene, 17(11), 1447-1455.