Based on the unexceptionable membrane protein production platform established through years of experience, scientists from Creative Biostructure provide tailored Mempro™ PGBD-like superfamily production services using detergent-free expression system.PGBD-like superfamily is made up of two families: Peptidoglycan binding domain (PGBD) family and MMP N-terminal domain family. Proteins of MMP N-terminal domain family are Fibroblast collagenase (MMP-1), Gelatinase A (MMP-2), Gelatinase B (MMP-9) and Stromelysin-1 (MMP-3). Proteins of Peptidoglycan binding domain(PGBD) family are N-acetylmuramoyl-L-alanine amidase YbjR (C-terminal domain) and Zn2+ DD-carboxypeptidase (N-terminal domain). Structurally similar PGBD-like domains may probably be discovered in Eukaryotic enzymes. The N-terminal domains of Matrix metalloproteinases (MMP) are similar to PGBD, besides, MMPs accelerate extracellular matrix degradation. For example, MMP-2, which is closely related to arthritis and tumour invasion, degrades MMP-3, a type IV collagen. A number of MMPs are involved in cancer progression, because degradation of the extracellular matrix is a crucial step in the cascade of metastasis.
Figure 1. Structure of Streptomyces Albus muramoyl-pentapeptide carboxypeptidase. (PMID 1998)
Creative Biostructure has ample professional experience in high quality PGBD-like superfamily production using detergent-free membrane protein expression system, we can perform various strategies for Mempro™ detergent-free protein production, including:
Nanodiscs possibly enable to maintain membrane proteins in solution, and offer an inartificial nature phospholipid bilayer environment in which the incorporated target keeps stably. It has been indicated that Nanodiscs enable to deliver porphyrin-conjugated lipid in a fallow form.
Amphipols (APols) meaning small amphipathic polymers which are enable to hold individual MPs water-soluble in their native state under the form of little hydrophilic complexes. Amphipols can be characteristically used in biophysics and biochemistry by the physicochemical properties of amphipols cut, which is based on the wide resources of polymer chemistry.
This method generates SMA lipid particles in which a little disk of lipid bilayer revolved around by polymer encircles the membrane proteins. SMA copolymer consists of a polymer mixed by maleic anhydride and styrene in all kinds of proportion.
These novel detergent-free approaches for PGBD-like superfamily production can be obtained easily, and enabling more comprehensively structural and functional studies.
Creative Biostructure provides other various Mempro™ membrane protein production services. Please feel free to contact us for a detailed quote.
References: Dideberg, O., Charlier, P., Dive, G., Joris, B., Frère, J.-M., & Ghuysen, J.-M. (1982). Structure of a Zn2+-containing D-alanyl-D-alanine-cleaving carboxypeptidase at 2.5 A resolution. Nature, 299, 469-470. Krogh, S., Jørgensen, S. T., & Devine, K. M. (1998). Lysis Genes of the Bacillus subtilisDefective Prophage PBSX. Journal of bacteriology, 180(8), 2110-2117. Jamshad, M., Lin, Y.-P., Knowles, T. J., Parslow, R. A., Harris, C., Wheatley, M., . . . Overduin, M. (2011). Surfactant-free purification of membrane proteins with intact native membrane environment. Biochemical Society Transactions, 39(3), 813-818.