Based on the comprehensive membrane protein production platform established through years of experience, Creative Biostructure can offer first-class custom Mempro™ C2 domain-containing protein production services based on plant-based expression system.
Creative Biostructure has the patent Mempro™ plant-based protein production platform, from which provides recombinant membrane proteins with animal-free, low endotoxin, and low protease activity. Most notably, membrane proteins expressed by plant system are free of animal components, serum, endotoxins and antibiotics as well as human or animal infectious agents or other endogenous mammalian contamination.
Creative Biostructure provides membrane protein production services based on various types of plants, such as Nicotiana benthamiana (tobacco), Medicago rativa (alfalfa), Arabidopsis thaliana (A. thaliana), potato, maize, barley and lettuce. Owing to plant-derived membrane protein production platform has lots of significant advantages, such as cost-effective, high yield, easy storage and distribution, friendly environment, and free of infectious or toxic contaminants, this system can be widely used in recent years.
C2 domain is a Ca2+-binding motif of approximately 130 amino acids in length, which involves in targeting proteins to cell membranes in eukaryotes. The first identification of C2 domain is the Ca2+-dependent isoforms of protein kinase C. It has been shown that C2 domain frequently couples with enzymatic domains (such as phosphatase domain). The signifcant feature of C2 domain is that it has a wide range of lipid selectivity for the major components of eukaryotic cell membranes, including phosphatidylserine and phosphatidylcholine. Most notably, C2 domain shows the property of binding a wide range of lipids, such as phospholipids and inositol polyphosphates.
Figure 1. The structural model of C2 domain of abscisic acid-related protein CAR4. (OPM Database)
With the Mempro™ plant-based membrane protein production platform, Creative Biostructure is able to express, isolate, purify and crystallize C2 domain-containing proteins to facilitate the study of their biological functions. Misfolding, aggregation, inactivity, poor stability and solubility, etc. are the common difficulties encountered in cell-based expression system. Strikingly, these difficulties can be bypassed in the renewed plant-based expression system.
Creative Biostructure can also provide Mempro™ plant-based virus-like particles (VLPs) production services and Mempro™ membrane protein production services. Please feel free to contact us for a detailed quote.
References:A. Wiktorek-Smagur, et al. (2012). Green way of biomedicine – how to force plants to produce new important proteins. Transgenic Plants-Advances and Limitations, Chapter 3. doi: 10.5772/1409. E. A. Nalefski and J.J. Falke (1996). The C2 domain calcium-binding motif: structural and functional diversity. Protein Sci., 5(12): 2375-2390.F. Junge, et al. (2008) Large-scale production of functional membrane proteins. Cellular and molecular life sciences, 65 (11): 1729-1755.