Creative Biostructure can offer advanced and tailored Mempro™ peptide fragment of transmembrane protein production services based on plant-based expression system for further structural analysis.
Our patent Mempro™ plant-based protein production system is able to generate animal-free, low endotoxin, and low protease activity membrane proteins. Notably, membrane proteins expressed by plant system are free of animal components, serum, endotoxins and antibiotics as well as human or animal infectious agents or other endogenous mammalian contamination. Additionally, post-translational modification such as glycosylation and disulfide bonds can be introduced in plant system. Thus, membrane protein production based on plant system is an innovative platform in recent years.
Creative Biostructure provides membrane protein production services based on a large range of host plants, such as Nicotiana benthamiana (tobacco), Medicago rativa (alfalfa), Arabidopsis thaliana (A. thaliana), potato, maize, barley and lettuce.
The significant advantages of plant-derived membrane protein production include:
Transmembrane proteins differ from the water-soluble proteins via highly hydrophobic segments required for membrane insertion. However, this inherent hydrophobicity and low expression of transmembrane proteins have hindered the study of structural information of membrane proteins. Thus, individual transmembrane fragments or segments derived from membrane protein folding have been observed to represent independent folding domains. Creative Biostructure can optimize synthesis and expression of peptide fragments of transmembrane proteins to facilitate the study of packing interactions between transmembrane protein helices, and the collection of structural information regarding integral membrane proteins.
Figure 1. The schematic diagram of seven transmembrane regions of β adrenergic receptors (βAR). (D. Sanz-Rosa, 2011, doi: 10.1016/j.hipert.2011.02.002)
With the Mempro™ plant-based membrane protein production platform, Creative Biostructure can express, isolate, purify and crystallize peptide fragments of transmembrane proteins to facilitate the study of their structural and biological functions.
Creative Biostructure can also provide Mempro™ plant-based virus-like particles (VLPs) production services and Mempro™ membrane protein production services. Please feel free to contact us for a detailed quote.
References:A. Perdih, et al. (2012). Structural analysis of a peptide fragment of transmembrane transporter protein bilitranslocase. PLoS ONE, 7(6): e38967.A. Wiktorek-Smagur, et al. (2012). Green way of biomedicine – how to force plants to produce new important proteins. Transgenic Plants-Advances and Limitations, Chapter 3. doi: 10.5772/1409. J. Venkatraman, et al. (2002). Structural analysis of synthetic peptide fragments from emre, a multidrug resistance protein, in a membrane-mimetic environment. Biochemistry, 41(21): 6631-6639.