Creative Biostructure offers dedicated services aimed to determine the structure of proteins, nucleic acids and their complexes. With our state-of-the-art facilities, we have developed an all-in-one pipeline including all the stages from construct design to structure determination. Our experienced scientists can teamwork with customers and accelerate the research progress.
Figure 1. Various structures of proteins
Currently more than 115,000 protein structures have been deposited into the Protein Data Bank (PDB) and this number keeps growing at an unprecedented speed due to technical advances over the years. Several methods are used to determine the structure of a protein, including X-ray crystallography, NMR spectroscopy, and electron microscopy (EM). X-ray crystallography has made the largest contribution to the number of protein structures deposited in PDB. NMR excels at the determination of protein conformations and study of protein dynamics in aqueous solutions. EM, and particularly Cryo-EM can be used to study relatively large objects such as macromolecular assemblies and complexes.
Creative Biostructure takes advantage of the above three methods and provides relevant services for your structure determination projects.
We use a variety of methods to determine phases and build electron density maps, including molecular replacement, multiple isomorphous replacement and single- or multi-wavelength anomalous dispersion.
We offer our expertise in NMR spectroscopy to accelerate your research in high-resolution protein structure determination in solution, as well as ligand binding assay and fragment screening.
We have an all-in-one cryo-EM pipeline from sample preparation, imaging data processing to final model building for large macromolecules and their complexes with small ligands, proteins and nucleic acids.
Please feel free to contact us to discuss your project!