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MASA Protein Crystal(CBCRY26)

Product Summary
Name MASA Protein Crystal Download Datasheet
Background
Background Enolase-phosphatase E1 (MASA) is a bifunctional enzyme in the ubiquitous methionine salvage pathway that catalyzes the continuous reactions of 2,3-diketo-5-methylthio-1-phosphopentane to yield the aci-reductone metabolite using Mg2+ as cofactor. In this study, we have determined the crystal structure of MASA and its complex with a substrate analog to 1.7A resolution by multi-wavelength anomalous diffraction and molecular replacement techniques, respectively.
Molecular Description
Protein ClassificationHydrolase
Structure Weight29316.11 Da
Polymer1
MoleculeE-1 Enzyme
Chain Length285 amino acids
Crystal Description
PDB ID1ZS9
MMDB ID33918
SourceE.coli
MethodX-Ray Diffraction
Resolution1.7Å
Ligand Chemical ComponentSelenomethionine
Gene Information
Gene NameENOPH1
SynonymsDKFZp586M0524; E1; FLJ12594; MASA; MST145; E-1 enzyme; Enolase-phosphatase E1; MSTP145 protein; acireductone synthase; EC 3.1.3.77; 2,3-diketo-5-methylthio-1-phosphopentane phosphatase; MASA homolog
UniProt IDQ9UHY7
Gene ID58478
Chromosome Location4q21.22
Functionmagnesium ion binding; phosphor-glycolate phosphatase activity; acireductone synthase activity; metal ion binding; catalytic activity
Reference
Reference Wang, H., Pang, H., Bartlam, M., Rao, Z. (2005) Crystal Structure of Human E1 Enzyme and its Complex with a Substrate Analog Reveals the Mechanism of its Phosphatase/Enolase J.Mol.Biol. 348: 917-926

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